Low-input RNA seq assesses the transcriptome in challenging sample types where there is a small amount of cells, tissue, or RNA available.
Low Input RNA Sequencing
It allows you to detect significant changes in gene expression for low-abundance transcripts and discover biomarkers and transcription factors. BGI offers low-input RNA-seq services with DNBSEQ technology at low cost.
Library preparation can be done with >200pg RNA, and SPIA (single primer isothermal amplification) technology can be selected according to sample quality results. The service can be useful for low RNA samples such as exosomal samples.
- 100bp pair-end sequencing reads
- Low RNA Input library preparation
- Clean data and bioinformatics analysis are available
in standard file formats - Available data storage and bioinformatics applications
- Cloud-based data storage and delivery system
| low amount + good quality | 200pg-50ng total RNA , High quality RNA samples (RIN>6.5, 28S/18S≥1.0,Not contaminated with DNA, protein or salt ions) |
| low amount + bad quality | >1ng total RNA, SPIA technology. |
- Please contact us.
DNBSEQ™ Sequencing Technology
BGI’s RNA Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.
