circRNAs are a novel class of abundant, stable and ubiquitous RNAs. circRNAs are distinguished from mRNAs in that they lack poly (A) tails and 5ʹ caps, and are resistant to exonuclease treatment. circRNAs can serve as miRNA or RNA-binding protein ‘sponges’, sequestering miRNAs and preventing their interactions with target mRNAs, as a result, controlling transcriptional events.
circRNA Sequencing
Circular RNAs (circRNAs) are produced from precursor mRNA (pre-mRNA) back-splicing of thousands of genes in eukaryotes. Although circRNAs are generally expressed at low levels, recent findings have shed new light on their cell type-specific and tissue-specific expression and on the regulation of their biogenesis. Furthermore, the data indicate that circRNAs shape gene expression by titrating microRNAs, regulating transcription and interfering with splicing, thus effectively expanding the diversity and complexity of eukaryotic transcriptomes.
In addition to the circular RNAs that are formed from thousands of loci in eukaryotes by exon back-splicing circularization, different types of circular RNAs exist, which are produced by distinct mechanisms. First, circular viral RNA genomes are ligated by host cellular enzymes to form 3ʹ,5ʹ-phosphodiester bonds or 2ʹ,5ʹ-phosphodiester bonds.
Second, circular RNA intermediates can be generated during rRNA processing and permuted tRNA biogenesis in Archaea and algae. Third, a number of housekeeping non-coding RNAs, including small nucleolar RNAs and the ribozyme RNase P, were also identified in circular forms in Archaea. Finally, various circular RNAs that are produced from spliced introns and exons have been reported. For example, excised, self-spliced group I and group II introns are ligated to form circular RNAs with either 3ʹ,5ʹ-phosphodiester bonds or 2ʹ,5ʹ-phosphodiester bonds. Excised tRNA introns are removed by tRNA splicing enzymes and then undergo 3ʹ,5ʹ-phosphodiester ligation in Archaea and Metazoa. Circular RNAs derived from lariat introns have been reported in human cells and in Xenopus tropicalis
- 150bp pair-end sequencing reads
- rRNA depletion and RNase linearRNA digestion library service
- Standard output 30-40 Million reads per sample
- Clean data and bioinformatics analysis are available
in standard file formats - Available data storage and bioinformatics applications
- Cloud-based data storage and delivery system
| Total RNA of Animals | Strongly Recommended | 15 μL – 100 μL | m≥10 μg | c≥40ng/μL, RIN≥7.0, 28S/18S≥1.0 |
| Required | 5μg≤m<10μg | |||
| Total RNA of Plant | Strongly Recommended | 15 μL – 100 μL | m≥10 μg | c≥40ng/μL, RIN≥6.5, 28S/18S≥1.0 |
| Required | 5μg≤m<10μg |
- Quality control and statistics of Reads
- Mapping to genome
- Localization of circRNA on genome
- Overview of Statistics of circRNA typies
- FPKM Interval (FI) of circRNA expression
- Density of circRNA expression
- CircRNA expression profiling
- Statistics of back splicing junctions
- Profiling of differentially expressed circRNA
DNBSEQ™ Sequencing Technology
BGI’s RNA Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.
