PTM Proteomics

PTM Proteomics

Post-translational modification (PTM) of proteins refers to the chemical changes proteins may undergo after translation. Common PTMs include Phosphorylation, Glycosylation, Acetylation and Ubiquitination. As a result, identifying and understanding PTMs is key for the study of cell biology and the treatment and prevention of disease.

BGI Genomics has extensive experience in the field of PTM Proteomics1,2 and has developed reliable workflows using market leading technologies and an advanced bioinformatics infrastructure.

Mass Spectrometry Service Specification

PTM Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X and Orbitrap Fusion Lumos).

Sample Preparation and Services

Digestion performed using sequencing-grade trypsin

Off-line sample fractionation using HPLC technology

Each fraction analyzed using nano-flow LC-MS/MS

TiO₂/ZrO₂ enrichment for phosphoproteomics; CST antibody enrichment for acetylated/ ubiquitylated proteins; MAX column enrichment for glycosylated proteins

Quality Standard

Summary includes all methods and data analysis

Reports provided in Excel or PDF format, RAW files available upon request

Turn Around Time

Phosphorylation Turn Around Time from sample QC acceptance to data report delivery 3-5 weeks

Glycosylation Turn Around Time from sample QC acceptance to data report delivery 4-5 weeks

Acetylation/Ubiquitylation Turn Around Time from sample QC acceptance to data report delivery 3-5 weeks

PTM modulates protein activity and macromolecular interactions and is involved in a range of fundamental molecular processes. Common PTMs include Phosphorylation, Glycosylation, Acetylation and Ubiquitination. As a result, identifying and understanding PTMs is key for the study of cell biology and the treatment and prevention of disease.

Phosphorylation is the most common mechanism of regulating protein function and transmitting signals throughout the cell.

Glycosylation is the reaction in which a carbohydrate is attached to a hydroxyl or other functional group of another molecule, and is key for molecular recognition,signal transduction and immune respons.

Acetylation describes a chemical reaction that introduces an acetyl functional group into a chemical compound and is involved with gene expression regulation, chromatin structure, DNA damage repair and cancer development.

Ubiquitylation plays an important role in localization, metabolism, function, regulation, and degradation, and is closely related to the occurrence of diseases such as tumors and cardiovascular diseases.

Specifications

  • Digestion performed using sequencing-grade trypsin
  • Off-line sample fractionation using HPLC technology
  • Each fraction analyzed using nano-flow LC-MS/MS
  • TiO₂/ZrO₂ enrichment for phosphoproteomics; CST antibody enrichment for acetylated/ ubiquitylated proteins; MAX column enrichment for glycosylated proteins
  • Summary includes all methods and data analysis
  • Reports provided in Excel or PDF format, RAW files available upon requ
  • Phosphorylation Turn Around Time from sample QC acceptance to data report delivery 3-5 weeks
  • Glycosylation Turn Around Time from sample QC acceptance to data report delivery 4-5 weeks
  • Acetylation/Ubiquitylation Turn Around Time from sample QC acceptance to data report delivery 3-5 weeks

Sample Requirements

Please see the catalogue

Data Analysis

  • Data analysis and validation performed with Mascot
  • Protein PTM identification
  • Protein PTM quantification
  • Modified protein function annotation
  • Differential modified protein function enrichment
  • Kinase prediction analysis
  • Motif distribution of PTM sites

PTM Proteomics

Unique DNBSEQ™ Sequencing Technology

BGI’s Single Cell Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ can offer advantages in terms of lower amplification error rates and much lower duplication rates for some sequencing applications. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.