Long Read Sequencing

BGI’s PacBio sequencing services are executed on PacBio’s Sequel II and Revio platformes. These platforms have been recognized for its ability to generate longer reads with greater accuracy and throughput, at a significantly lower cost.

Our long-read sequencing services are performed with the PacBio Revio platform and the Nanopore platform.

PACBIO REVIO PLATFORM

 

  • With high density, 25 million ZMW SMRT (Single Molecule, Real-time) cells, up to 4 SMRT cells sequencing in parallel and optimized on-instrument base calling, the PacBio Revio sequencer delivers up to 360 Gb of HiFi data in less than 24 hours.
  • Revio offers HiFi reads by default, which are tens of kb long, with 90% of bases >Q30, providing the ability to resolve large variants and traditionally difficult to map, repetitive regions of the genome. HiFi sequencing also helps discover DNA methylation, without bisulfite treatment.
  • BGI delivers unmatched data output while maintaining a mean data quality of >Q30, owing to its extensive experience with sequencing samples from different species. For samples that pass our QC requirements, also offers guaranteed data output on Revio.

PACBIO SEQUEL II/IIE PLATFORM

 

 

  • The Sequel IIe is based on Single Molecule, Real-Time (SMRT) technology for long read DNA sequencing. Each Sequel IIe SMRT 8M flow cell has 8 million ZMW wells and can generate up to 30 Gb HiFi data per flow cell.
  • The platform offers two different sequencing modes: CLR (legacy) for accurate, long reads with an emphasis on obtaining the longest reads possible and CCS (HiFi) with an emphasis on obtaining long reads with high accuracy through multiple passes of the same molecule.
  • BGI has extensive sequencing experience for samples belonging to a broad range of species on the Sequel IIe and therefore continues to deliver excellent data quality.

Specifications

  • Sequel IIe system Sequel II system Sequel system
    Supported SMRT Cell SMRT Cell 8M SMRT Cell 8M SMRT Cell 1M
    Number of HiFi reads >99%* accuracy Up to 4,000,000 Up to 4,000,000 Up to 500,000
  • PacBio Revio with SPRQ SBS sequencing Nanopore sequencing
    Read length

    15-20 kb5

    		 2×150 bp 10–100 kb
    Read accuracy

    99.95% (Q33)1

    99.92% (Q31)3

    98.90% (Q19)4
  • Sample Type Library Type Amount OD
    Genomic DNA PacBio HiFi CCS m≥14 μg, c≥80 ng/μL OD260/280：1.6-2.2 OD260/230：1.6-2.5
    Genomic DNA Normal Nanopore library m≥2 μg, c≥50 ng/μL OD260/280=1.8-2.0 OD260/230≥1.5
    Genomic DNA Nanopore Ultra long library m≥10 μg, c≥50 ng/μL OD260/280=1.8-2.0 OD260/230≥1.5

Sample Requirements

Please concact us

Standart Bioinformatics Analysis

Customized Bioinformatic Solutions

Long-read_Human_WGRS_Service_Overview

Unique DNBSEQ™ Sequencing Technology

BGI’s sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.