16S/18S/ITS Sequencing

We provide professional, fast and affordable 16S/18S/ITS gene sequencing services for the identification, classification and quantitation of microbes within complex biological mixtures. Contact us today with any questions or for a no obligation quote.

16S and 18S rDNA are hypervariable regions in the 16S or 18S rRNA genes in bacteria and fungus, while ITS (Internal Transcribed Spacer) is the spacer DNA between the small-subunit and large-subunit rRNA genes in bacteria, fungi and archaea.

Sequence comparison of 16S/18S/ITS regions is widely used in taxonomy and molecular phylogenetics because these regions have a high degree of variation even between closely related species, while also offering easy amplification by PCR, even from low quantities of DNA.

We have extensive experience supporting partners across the world with their 16S, 18S rDNA and ITS research needs, while our economies of sequencing scale and proprietary technology ensures extremely efficient pricing.

Specifications

  • PE300 sequencing by DNBSEQ
  • Up to ≥ 80% of bases with a ≥Q30 quality score, depending on the chosen sequencing strategy
  • Recommended sequencing coverage is dependent on the complexity of the sample

Sample Requirements

Genomic DNA – ≥50ng – ≥6ng/µL

PCR Fragment – ≥3µg – ≥30ng/µL

Standart Bioinformatics Analysis

  • Data filtration
  • Overlap paired-end reads to form tags
  • Tags are clustered into OTU. PCA, Venn diagram. A rank curve will be generated based on OTU abundance
  • Species are classified by OTU annotation, based on which a species profiling histogram, heat map and a phylogeny tree will be provided
  • Alpha diversity indexes are generated for in single sample
  • Beta diversity and clustering analysis are performed for multiple samples
  • Comparative analysis is used to screen significant differences in multiple samples

16S18SITS Amplicon Sequencing

Unique DNBSEQ™ Sequencing Technology

BGI’s 16S/18S/ITS Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms