Protein Identification

Service Description

Modern liquid chromatography and mass spectrometry technology provides a means to routinely perform non-targeted protein identification. BGI can help simplify the challenge of protein identification for your project.

Mass Spectrometry Service Specification

Protein Identification services are performed using nano-flow liquid chromatography and high resolution Orbitrap mass spectrometry.

  • Sample Preparation and Services

    • Digestion performed using sequencing-grade trypsin
    • 60 min nano-flow LC-MS/MS using a Q Exactive HF-X Orbitrap mass spectrometer

  • Mass Spec Services Quality Standard

    • Summary including all methods and data analysis
    • Reports provided in Excel or PDF format, RAW files available upon request

  • Turn Around Time

    • Typical 3-4 weeks from sample QC acceptance to data report delivery
    • Prime Expedited Services with 1 week Turn Around time is available

In-Gel Workflow

In-Gel Identification

We can identify proteins isolated in gel bands or spots. Our service includes destaining, in-gel digestion, nano-flow LC-MS/MS, and peptide spectral match data analysis.

In-Solution Workflow

In-Solution Identification

Our in-solution protein identification services can be used for applications such as monitoring recombinant protein expression and affinity purification systems.

Specifications

  • We accept protein samples in solution or gel format. For complex in-solution samples we can provide electrophoresis services, including preparative SDS-PAGE and analytical staining.

Sample Requirements

Sample type Amount and Concentration Minimum sample volume
Protein sample in liquid solution or gel band/spot Recommended 100 µg; 2 µg/µL or above 10 µL

 

Advanced Bioinformatics Analysis

  • Data analysis and validation performed with SEQUEST or Mascot
  • Top protein identifications, peptide spectral match count, and sequence coverage provided
  • Up to 30 proteins ranked by peptide spectral match count

Protein Identification Service Overview

 

Unique DNBSEQ™ Sequencing Technology

BGI’s RNA Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.