Long non-coding RNAs (lncRNAs) are a large class of transcribed RNA molecules with a length of more than 200 nucleotides that do not encode proteins. Contact us today with any questions or for a no obligation quote.
Long Non-Coding RNA Sequencing
Long non-coding RNAs (lncRNAs) are thought to encompass nearly 30,000 different transcripts in humans, hence lncRNA transcripts account for the major part of the non-coding transcriptome. lncRNA discovery is still at an early stage and only a small proportion of lncRNAs have so far been investigated. Although we can start to classify different types of lncRNA functions, we are still far from being able to predict the function of new lncRNAs.
BGI offers expression profiling as one way to uncover the function of lncRNA. Identifying lncRNAs that are differentially expressed during development or in particular situations can shed light on their potential functions. Alternatively, looking for lncRNAs and protein-coding genes whose expression is correlated, can indicate co-regulation or related functions.
- 100bp pair-end sequencing reads
- Strand specific library with rRNA removal
- Data and bioinformatics analysis are available in standard file formats
- Total RNA Amount ≥ 200ng – RIN ≥ 7,0
- Concentration ≥20ng/μl – 28S/18S≥1,0
- Identification of mRNAs and LncRNAs
- Quantification and differential expression analysis
- Gene ontology analysis and pathway enrichment analysis
Unique DNBSEQ™ Sequencing Technology
BGI’s Long Non-Coding RNA Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.
