Targeted Proteomics

Mass Spectrometry Service Specification

Targeted Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF/HF-X and QTRAP 6500).

Sample Preparation and Services

Customized LC method and MRM/PRM-acquisition MS method

Quality Standard

Summary includes all methods and data analysis

Reports provided in Excel or PDF format, RAW files available upon request

Turn Around Time

Typical 8 weeks from sample QC acceptance to data report delivery

Service Description

  • With the advancement of proteomics research, a large number of functional proteins and potential disease protein markers have been identified. However, for accurate and verifiable results and to ensure the significance of any findings, higher sample volumes are needed for large-scale quantitative research.
  • Traditional target protein analysis is based on antibody techniques, which have limitations in throughput and specificity.
  • However, parallel reaction monitoring (PRM)* approaches are able to resolve these obstacles. As the gold standard for targeted proteomics research based on mass spectrometry (MS) platforms, PRM technology can quickly and accurately determine dozens of proteins simultaneously in complex biological samples and solve slow verification problems.

Comparison of targeted MS methodology to antibody techniques.

Specifications

  • Customized LC method and MRM/PRM-acquisition MS method
  • Summary includes all methods and data analysis
  • Reports provided in Excel or PDF format, RAW files available upon request
  • Typical 8 weeks from sample QC acceptance to data report delivery

Sample Requirements

Please see the catalogue

 

Data Analysis

  • Summary of target proteins
  • Data quality
  • Method demonstration
  • Quantitative information
  • Data normalizatio
  • Quantification of target peptide

Targeted Proteomics

Unique DNBSEQ™ Sequencing Technology

BGI’s Single Cell Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ can offer advantages in terms of lower amplification error rates and much lower duplication rates for some sequencing applications. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.