Peptidomics

Service Description

Bioactive polypeptides are polypeptides with multiple biological functions, including signal molecules (such as cytokines), antimicrobial peptides and other small protein fragments, as well as peptides with undetermined functions or from disease-specific enzyme digestion.
Peptidomics is an emerging field filling the research gap between proteomics and metabolomics and can be defined as the comprehensive qualitative and quantitative analysis of all endogenous peptides from biological samples at a specific time and location. infrastructure.

Peptidomics analysis employs many proteomics techniques but with a different target. Rather than examining intact proteins in a sample, peptidomics detects endogenous protein fragments. The research object of peptidomics are the short peptides (with molecular weight less than 10 kDa) secreted extracellularly after processing of a precursor protein inside the cell, or the wide range of polypeptides produced by the further hydrolysis of extracellular macromolecular proteins.

Endogenous peptides in vivo exist in various body fluids. These low molecular weight peptides record a series of enzyme-catalyzed reactions in and out of the cell in the microenvironment of tumor tissue and reflect the related molecular mechanisms, which can be used as a rich resource for tumor specific diagnosis.
BGI has extensive experience in the field of peptidomics spanning disease biomarker study, off target effects of drugs, food processing and fermentation research, the molecular mechanisms of animals and plants, and cell antigen epitopes prediction.

Research Applications

• Disease biomarkers research
• Study of off target effects of peptide drugs
• Study of the molecular mechanisms of animals and plants
• Food processing and fermentation research
• Cell antigen epitopes prediction

Specifications

  • Enrichment of endogenous polypeptides
  • 1h for LC-MS/MS analysis
  • Summary includes all methods and data analysis
  • Reports provided in Excel or PDF format, RAW files available upon request
  • Typical 4-5 weeks from sample QC acceptance to data report delivery

Sample Requirements

Please see the catalogue

Data Analysis

  • Data analysis and validation performed with Mascot
  • Identification of peptides and proteins
  • Peptide quantification
  • Protein GO/COG/Pathway annotation
  • Identified peptide matching analysis of specific protein

Peptidomics

Unique DNBSEQ™ Sequencing Technology

BGI’s Single Cell Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ can offer advantages in terms of lower amplification error rates and much lower duplication rates for some sequencing applications. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.