BGI’s plant and animal whole genome sequencing, also referred to as whole genome re-sequencing (WGRS) involves sequencing the entire genome of a plant or animal, and comparing the sequence to that of a known reference genome. Re-sequencing of the plant and animal genome will identify genetic variations such as SNPs and InDels, and is often applied for the identification of functional genes and markers of important traits to facilitate molecular breeding and to improve agricultural production and conservation.
Whole Genome Re-Sequencing
WGS provides a more comprehensive, accurate and efficient tool for plant and animal genomics projects that traditionally have used legacy technology such as genotyping arrays or genotyping-by-sequencing. The WGS approach outperforms these methods by providing an order of magnitude more data, greater statistical power, and enhanced variant discovery capabilities, easier analysis – all at a lower cost.
Our end-to-end workflow can support you with a broad range of species. We also evaluate and accept custom projects on a case-by-case basis. We can perform deep sequencing for a select number of individuals and build a well assembled reference genome for imputations.
- PCR and PCR-Free library methods both available
- 100bp and 150bp paired-end sequencing available
- Raw data, standard and customized data analysis
- Available data storage and bioinformatics applications
- Guaranteed ≥85% of bases with a quality score of Q30
- Standard sequencing coverage 5 – 10X for population studies
- Standard sequencing coverage 10 – 30X for individual study
Regular Samples:
PCR
– Mass: ≥200ng (Recommend ≥400ng)
– Concentration: ≥8ng/μL
PCR-free
– Mass: ≥1μg (Recommend ≥2μg)
– Concentration: ≥12.5ng/μL
Low Input Samples:
PCR
– Mass: ≥50ng
– Concentration: ≥2.5ng/μl
- Data Filtering
- Alignment
- SNP/InDel/SV/CNV calling, annotation and statistics
Unique DNBSEQ™ Sequencing Technology
BGI’s sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.
