High-throughput targeted metabolomics HM Pro2300

Service Description

BGI provides one of the world’s most comprehensive panels for targeted metabolomics research. The HM Pro2300 panel uses LC-MS/MS technology for high-throughput targeted metabolomics detection. HM Pro2300 can simultaneously detect more than 2,000 metabolites, including 700 small molecules and more than 1,600 lipids which covers all kinds of important metabolites and core metabolic pathways. We also provide HM700 and HML1600, only detecting small molecules and lipids respectively.
HM700 includes more than 400 metabolites related to intestinal flora, which applies to address key pathophysiological processes relevant in gut microbiota research and cancer research areas. Each metabolite is quantitatively determined using the standard curve. This enables greater in-depth mining of metabolome information, providing accurate quantification, excellent reproducibility and high throughput.
The HML1600 can achieve absolute quantification for more than 1,600 lipids of 12 subclasses by the method of combining standard curves with internal standard, covering all kinds of lipids with important biological functions.
HML1600 is used for nervous system disease research, cardiovascular and cerebrovascular disease research, nutritional metabolism research, lipid metabolism research and other fields.

Mass Spectrometry Service Specification

HM Pro2300 high-throughput targeted metabolomics services are performed using Waters ACQUITY UPLC and SCIEX QTRAP 5500, 6500+.

Sample Preparation and Services

Custom-developed UPLC-MRM/MS methods combine isotope internal standard technology

Small molecules and lipids are extracted respectively to increase metabolome depth

Using SCIEX QTRAP 6500+, 5500 detect respectively small molecules and lipids to increase the scope of detection

Quality Standard

Summary includes all methods and data analysis

Reports provided in Excel or PDF format

Turn Around Time

Typical 6-8 weeks after sample receipt

Product Category of Metabolites Number of Metabolites
HM Pro2300 HM700-meta Amino acids and Peptides ~111
Fatty acids ~103
Organic acids and derivatives ~56
Bile acids ~111
Carbohydrates ~49
Benzenoids ~75
Carnitines or Acyl carnitines ~17
Indoles and derivatives ~23
Nucleosides ~22
Steroids and steroid derivatives ~9
Phenylpropanoids and polyketides ~37
Organooxygen ~11
Other ~76
HML1600-Lipids Glycerophospholipids ~1091
Glycerolipids ~513
Sterol Lipids ~26
Sphingolipids ~70
Total 2,300+

Specifications

  • Custom-developed UPLC-MRM/MS methods combine isotope internal standard technology
  • Small molecules and lipids are extracted respectively to increase metabolome depth
  • Using SCIEX QTRAP 6500+, 5500 detect respectively small molecules and lipids to increase the scope of detection
  • Summary includes all methods and data analysis
  • Reports provided in Excel or PDF format
  • Typical 6-8 weeks after sample receipt

Sample Requirements

SAMPLE TYPE RECOMMENDED SAMPLE AMOUNT MINIMUM SAMPLE AMOUNT
Serum, plasma, urine ≥ 200 µL ≥ 100 µL
Animal and clinical tissues, feces and intestinal contents, microorganism ≥ 100 mg ≥ 40 mg
Cell 2×10^7 2×10^5
Culture medium, fermentation medium ≥ 1 mL ≥ 200 µL

Data Analysis

  • Data analysis performed with metaX and BGI Genomics library
  • Data quality control
  • Standard curves
  • Quantification results

HM Pro2300


Unique DNBSEQ™ Sequencing Technology

BGI’s RNA Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.