Whole Genome Bisulfite Sequencing (WGBS) provides a scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells. Tell us about your project, our experts are here to help.
Whole Genome Bisulfite Sequencing
Methylation of DNA at the fifth position in cytosine (5-mC) is a stable epigenetic modification and plays an important role in many biological processes, including gene silencing, suppression of transposable elements, genomic imprinting and X chromosome inactivation. Detection and quantification of methylation are critical to understand gene expression and other processes subjected to epigenetic regulation.
Whole genome bisulfite sequencing (WGBS) is used to detect methylated cytosines by treating the DNA with sodium bisulfite before sequencing. WGBS has become the gold standard for studying genome-wide methylation at single base resolution.
- Library preparation, including bisulfite treatment
- 100bp paired-end sequencing
- Clean data, standard and customized data analysis
- Available data storage and bioinformatics applications
- Guaranteed ≥85% of clean bases with quality score of Q20
- Guaranteed ≥99% bisulfite conversion rate
- Standard sequencing coverage ≥30X is recommended
- Regular Samples – Intact genomic DNA ≥ 1μg, Concentration ≥ 50ng/μl – 15 µl
- Low Input Samples – Intact genomic DNA ≥ 100ng, Concentration ≥ 7ng/μ -15 µ
- Data Filtering
- Alignment
- Data Quality Statistics
- Methyl-cytosine identification
- Differential Methylated Region (DMR) analysis
- Statistics of methylation level
Whole Genome Bisulfite Sequencing Technology
BGI’s Whole Genome Bisulfite Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.
