Small RNA sequencing is used to discover novel small RNAs, examine the differential expression of all small RNAs and to characterize variations with single-base resolution. Contact us today with any questions or for a no obligation quote.
Small RNA Sequencing
Discover Our Unique Molecular Identifiers (UMIs) Service
Unique Molecular Identifiers (UMIs) can be utilized to eliminate undesirable PCR duplicates derived from a single molecule. After PCR, molecules sharing a UMI are assumed to be derived from the same input molecule. As such, UMI counts offer superior results to counting reads, leading to more accurate estimates of quantitative small RNA expression. UMI technology is especially beneficial to customers undertaking research on rare and precious samples or samples containing less RNAs, such as exosomes.
Small RNAs are a type of non-coding RNA (ncRNA) molecule that are less than 200nt in length. They are often involved in gene silencing and post- transcriptional regulation of gene expression. Our sequencing scale and extensive experience ensure high quality, fast small RNA servcies at unbeatable pricing.
- 50bp single-end sequencing reads
- Standard output 20 Million reads per sample
- UMI technology to enhance the quantification accuracy
- Sequencing data and bioinformatics analysis are available in standard file formats
- Advanced RNA data visualization and data mining with Dr.Tom system
- Guaranteed ≥80% of bases with quality score of ≥Q30
| RNA Amount and Concentration |
Minimum Sample Volume |
Quantitative Result | |
| Regular sample | mass ≥1μg Concentration ≥50ng/μl |
15 μl | RIN≥6.5 (plant) RIN ≥8.0 (human/animal) |
| FFPE RNA | mass ≥1μg Concentration ≥500ng/μl |
15 μl | RIN ≥2.0 DV200 ≥30% |
| Small RNA of Plasma/serum/exosome |
mass ≥20μg Concentration ≥1ng/μl |
15 μl | N/A |
- Data filtering
- Length distribution of small RNAs
- Analysis of common and specific sequences between two samples
- Small RNAs distribution across selected genome
- Identification of rRNAs, tRNAs, snRNAs, snoRNAs, etc.
- Identification of repeat associated small RNAs
- Identification of small RNA sequences which could align to exon/intron
- Identification of known miRNAs by aligning to designated part of miRBase
- Analysis of the expression pattern of known miRNAs
- Classification of small RNAs into several categories based on customer preference
- Prediction of novel miRNAs and their secondary structures by Mireap and miRDeep from unannotated small RNAs
- Family analysis of known miRNAs
Unique DNBSEQ™ Sequencing Technology
BGI’s sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.
