High-throughput targeted metabolomics HML1600

Service Description

BGI provides one of the world’s most comprehensive panels for targeted metabolomics research. The HML1600 performs high throughput targeted metabolomics detection and analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) quantitative analytical technique for more than 1,600 lipids of 12 subclasses by the method of combining standard curves with internal standard, covering all kinds of lipids with important biological functions. HML1600 is used for nervous system disease research, cardiovascular and cerebrovascular disease research, nutritional metabolism research, lipid metabolism research, and other fields.

Mass Spectrometry Service Specification

HML1600 high-throughput targeted metabolomics services are performed using Waters ACQUITY UPLC and SCIEX QTRAP 5500.

Sample Preparation and Services

Custom-developed UPLC-MRM/MS methods combine isotope internal standard technology

Metabolomics quantification using standard curves and internal standards with exceptional accuracy and precision

Using LC-MS (SCIEX QTRAP 5500) to increase the scope of detection

Quality Standard

Summary includes all methods and data analysis

Reports provided in Excel or PDF format

Turn Around Time

Typical 5-7 weeks after sample receipt

Category Main Class Sub Class Number of  Metabolites Representative Lipids
Glycerophospholipids
(GP)		 P-Choline Phosphatidylcholine(PC) ~211 PC 16:0-16:0, PC 16:0-16:1,
PC 16:0-18:0
Lysophosphatidylcholine (LPC) ~24 LPC 14:0, LPC 15:0,
LPC 16:0, LPC 16:1, LPC 17:0
P-Glycerol Phosphatidylglycerol (PG) ~211 PG 16:0-16:0, PG 16:0-16:1,
PG 16:0-18:0
P-Inositol Phosphatidylinositol (PI) ~210 PI 16:0-16:0, PI 16:0-16:1,
PI 16:0-18:0, PI 16:0-18:1
P-Serine Phosphatidylserine (PS) ~204 PS 16:0-16:0, PS 16:0-16:1,
PS 16:0-18:3, PS 16:0-18:4
P-Ethanol
Amine		 Phosphatidylethanolamine (PE) ~207 PE 16:0-16:0, PE 16:0-16:1,
PE 16:0-18:0, PE 16:0-18:1
Lysophosphatidylethanolamine (LPE) ~24 LPE 14:0, LPE 15:0, LPE 16:0,
LPE 16:1, LPE 17:0
Glycerolipids
(GL)		 Diradylglycerols Diacylglycerol (DAG) ~49 DAG 16:0-16:0, DAG 16:0-16:1,
DAG 16:0-18:0
Triacylglycerols Triacylglycerol (TAG) ~464 TAG 42:0-FA12:0,
TAG 42:0-FA14:0,
TAG 42:0-FA16:0
Sterol Lipids
(ST)		 Sterols Cholesterol Ester (CE) ~26 CE 12:0, CE 14:0, CE 14:1,
CE 15:0, CE 16:0
Sphingolipids
(SP)		 Ceramides Ceramides (CER) ~13 Cer d18:1/14:0, Cer d18:1/16:0,
Cer d18:1/16:1
Sphingolipids Sphingomyelin (SM) ~57 SM 34:0, SM 35:0
Total 1600+

Specifications

  • Custom-developed UPLC-MRM/MS methods combine isotope internal standard technology
  • Metabolomics quantification using standard curves and internal standards with exceptional accuracy and precision
  • Using LC-MS (SCIEX QTRAP 5500) to increase the scope of detection
  • Summary includes all methods and data analysis
  • Reports provided in Excel or PDF format
  • Typical 5-7 weeks after sample receipt

Sample Requirements

AMPLE TYPE RECOMMENDED SAMPLE AMOUNT MINIMUM SAMPLE AMOUNT
Serum, plasma, urine ≥ 100 µL ≥ 50 µL
Animal and clinical tissues, feces and intestinal contents, microorganism ≥ 50 mg ≥ 20 mg
Cell 1×10^7 1×10^5
Culture medium, fermentation medium ≥ 500 µL ≥ 100 µL

Data Analysis

 

Data Analysis

  • Data analysis performed with metaX and BGI Genomics library
  • Data quality control
  • Standard curves
  • Quantification results

HML1600


Unique DNBSEQ™ Sequencing Technology

BGI’s RNA Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.