Nanoproteomics

Nanoproteomics Service Overview

To date, most proteomics studies have been based on proteins obtained from the homogenization of large volumes of cells or tissues. However, with the advancement of medical research, there is a growing need for the analysis of highly specialized specimens within specific applications and research areas such as trace specimens identified by forensic experts, or intra-tumor heterogeneity research.
Nanoproteomics refers to quantitative proteomics analysis of small cell populations (typically < 5,000 cells) by the combination of in-situ cleavage and DDA label free technology, and offers possibilities for analysis unavailable with conventional protein extraction and mass spectrometry.
BGI has extensive experience in the field of nanoproteomics spanning cellular heterogeneity research across rare cell populations, hard-to-obtain clinical specimens, and pathological tissues.

Mass Spectrometry Service Specification

Nanoproteomics services are performed using nano-flow liquid chromatography and cutting-edge Orbitrap Fusion Lumos mass spectrometer.

Sample Preparation and Services

The QC of enzymatic hydrolysis is strictly controlled by LC-MS

DDA-Spectral Library generated using HPLC technology and sample fractionation

2h/fraction; 2h for DDA analysis

 

Quality Standard

Summary includes all methods and data analysis

Reports provided in Excel or PDF format, RAW files available upon request

Turn Around Time

Typical 5-6 weeks from sample QC acceptance to data report delivery

Workflow

 

Specifications

  • The QC of enzymatic hydrolysis is strictly controlled by LC-MS
  • DDA-Spectral Library generated using HPLC technology and sample fractionation
  • 2h/fraction; 2h for DDA analysis
  • Summary includes all methods and data analysis
  • Reports provided in Excel or PDF format, RAW files available upon request
  • Typical 5-6 weeks from sample QC acceptance to data report delivery

Sample Requirements

Please see the catalogue

Data Analysis

  • Data analysis and validation performed with Mascot
  • Protein identification and quantification list
  • Cluster analysis of expression patterns across multiple samples
  • Protein GO/COG/KOG/Pathway annotation
  • GO/Pathway enrichment analysis of differential proteins
  • Protein-protein interaction analysis
  • Protein subcellular localization analysis
  • Repeatability analysis
  • Quantitative proteomics and phosphoproteomics correlation analysis
  • Repeatability analysis
  • Quantitative proteomics and phosphoproteomics correlation anal

Nanoproteomics


Unique DNBSEQ™ Sequencing Technology

BGI’s RNA Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.