Transcriptome sequencing is used to reveal the presence, quantity and structure of RNA in a biological sample under specific conditions. Compared to hybridization-based RNA quantification methods such as microarray analysis, sequencing-based transcriptome detection can quantify gene expression with low background, high accuracy and high levels of reproducibility within a large dynamic range. In addition, transcriptome sequencing does not require an existing genome sequence and can detect, splice variants and fusion genes that cannot be detected by microarrays.
RNA Sequencing (Transcriptome)
Our world leading RNA Sequencing services are relied upon by researchers and companies all over the world. Our scale and experience enable us to offer industry beating pricing, highly efficient turnaround times and fantastic project support. Contact us today with any questions or for a no obligation quote.
RNA Sequencing, sometimes referred to as transcriptome sequencing, is used to reveal the presence, quantity and structure of RNA in a biological sample under specific conditions. Compared to hybridization-based RNA quantification methods such as microarray analysis, sequencing-based transcriptome detection can quantify gene expression with low background, high accuracy and high levels of reproducibility within a large dynamic range. In addition, transcriptome sequencing does not require an existing genome sequence and can detect splice variants and fusion genes that can not be detected by microarrays.
- Multiple choices for mRNA enrichment and rRNA removal kits
- Stranded sequencing
- 100bp and 150bp paired-end sequencing options available
- Raw data and bioinformatics analysis are available in standard file formats
- Advanced and custom bioinformatics data analysis
- Cloud-based data storage and delivery system
- >30M reads per sample recommended
| Total RNA | |||||
| Species | Amount | Concentration ng/μL |
RIN/RQN Value | 28S /16S | DV200 |
| Human/mouse/rat (non-whole blood) |
≥200ng | ≥10 | ≥7 | ≥1.0 | N/A |
| Human (whole blood) |
≥500ng | ≥40 | ≥7 | ≥1.0 | N/A |
| Human (FFPE) | ≥200ng | ≥70 | ≥2 | N/A | ≥30% |
| Insect | ≥1µg | ≥40 | N/A | N/A | N/A |
| Other Animals | ≥1µg | ≥40 | ≥7 | ≥1.0 | N/A |
| Plant | ≥1µg | ≥40 | ≥6 | ≥1.0 | N/A |
| Fungi | ≥1µg | ≥40 | ≥6,5 | ≥1.0 | N/A |
- Quantitative expression profiles
- Alternative splicing analysis
- Fusion gene analysis
- Time series analysis
- Pathway enrichment analysis
- Hierarchical clustering analysis
- Protein – Protein interaction (PPI) analysis
- Gene ontology analysis
Unique DNBSEQ™ Sequencing Technology
BGI’s RNA Sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.
