Cell populations are heterogeneous and unsynchronized in their characteristics. Traditional bulk RNA sequencing generates comprehensive information but does not provide insight into variable expression between cells. High throughput single cell RNA-Seq provides a scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells. High throughput single cell RNA-Seq technology is able to handle a large quantity of single cells fast and accurately, providing research solutions across a wide variety of applications including cancer, neurology, and immunology.
High Throughput Single Cell RNA-Seq
The major applications of single cell sequencing include:
- Discovering new cells and cell subtypes
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Studying tissue and cell group heterogeneity
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Studying tumor-immune cell interaction
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Searching for marker genes for drug development
- Guaranteed ≥90% of bases with quality score of ≥Q20
- DNBSEQ™ PE100 or PE150 options
- Raw data and bioinformatics analysis are available in standard file formats
Cell line/Tissue/PBMC:
- After sample processing, freeze cells at a final concentration of 1×10^6 cells/ml in cryopreserved tubes (Cell freezing medium: 90% FBS+10% DMSO)
- Sequencing Result
- Comparison Result
- Quantitative Analysis
- Data QC
- Cell Cluster
- Differential Gene Identification Between Samples
- Cell Cluster Annotation
- Marker Gene/Differentially Expressed Gene GO Pathway
- Marker Gene/Differentially Expressed Gene KEGG Pathway Fnrichment
- RNA Velocity
- CellPhone DB
- Pseudotemporal Ordering
- Customized Cell Cluster Analysis
- Customized Cell Cluster Annotation By Database of your choice
- Gene-Protein Interaction Analysis
- Gene Function Enrichment Analysis
- Dynamic Interactive Analysis of Cell Type And Cell Trajectory Analysis
- Different Dimension Analysis of Differential Gene Between Cell Types/Samples
Unique DNBSEQ™ Sequencing Technology
BGI’s sequencing services are typically executed with proprietary DNBSEQ™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ™ offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ™ platforms.
