{"id":4937,"date":"2025-01-06T15:51:20","date_gmt":"2025-01-06T15:51:20","guid":{"rendered":"https:\/\/www.geneethic.com.tr\/?page_id=4937"},"modified":"2025-01-07T09:00:44","modified_gmt":"2025-01-07T09:00:44","slug":"low-input-rna-sequencing","status":"publish","type":"page","link":"https:\/\/www.geneethic.com.tr\/?page_id=4937","title":{"rendered":"Low Input RNA Sequencing"},"content":{"rendered":"[vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}”][vc_column css=”.vc_custom_1684760689814{background-color: #f6f6f6 !important;}”][vc_row_inner][vc_column_inner animation=”animation bottom-to-top”]\t
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\n\t\t\t\t\t\"\"\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t<\/div>\n [vc_column_text css=”.vc_custom_1736240293457{margin-bottom: 0px !important;}”]Low-input RNA seq assesses the transcriptome in challenging sample types where there is a small amount of cells, tissue, or RNA available.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113590633{padding-top: 60px !important;background-color: #f6f6f6 !important;}”][vc_column][vc_row_inner et_column_align=”align-center” content_placement=”middle”][vc_column_inner width=”1\/2″ animation=”animation bottom-to-top” offset=”vc_col-lg-6″][vc_column_text css=”.vc_custom_1736240439601{margin-bottom: 0px !important;}”]It allows you to detect significant changes in gene expression for low-abundance transcripts and discover biomarkers and transcription factors. BGI offers low-input RNA-seq services with DNBSEQ technology at low cost.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″ animation=”animation bottom-to-top”][vc_column_text css=”.vc_custom_1736179620590{margin-bottom: 0px !important;}”]Library preparation can be done with >200pg RNA, and SPIA (single primer isothermal amplification) technology can be selected according to sample quality results. The service can be useful for low RNA samples such as exosomal samples.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner]\t\t
\n\t\t\tNeed a quotation?<\/span>\r\n\r\n\r\n<\/svg>\r\n<\/a>\n\t\t<\/div>\n\t\t\n\t[vc_row_inner et_max_width=”” et_column_align=”align-center” content_placement=”middle”][vc_column_inner offset=”vc_col-lg-6″][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_column_align=”align-center” css=”.vc_custom_1579113617984{padding-top: 60px !important;padding-bottom: 60px !important;}”][vc_column width=”1\/2″ animation=”animation bottom-to-top”][vc_tta_accordion active_section=”1″][vc_tta_section title=”Specifications” tab_id=”1557543124640-60cb9bb3-2cc8″][vc_column_text]\n
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  • 100bp pair-end sequencing reads<\/li>\n
  • Low RNA Input library preparation<\/li>\n
  • Clean data and bioinformatics analysis are available
    \nin standard file formats<\/li>\n
  • Available data storage and bioinformatics applications<\/li>\n
  • Cloud-based data storage and delivery system<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_tta_section][vc_tta_section title=”Sample Requirements” tab_id=”1557543124710-e0287025-a0eb”][vc_column_text]\n\n\n\n\n
    \u00a0low amount + good\u00a0 quality<\/td>\n200pg-50ng total RNA , High quality RNA samples (RIN>6.5, 28S\/18S\u22651.0,Not contaminated with DNA, protein or salt ions)<\/td>\n<\/tr>\n
    \u00a0low amount + bad quality<\/td>\n>1ng total RNA, SPIA technology.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][\/vc_tta_section][vc_tta_section title=”Standart Bioinformatics Analysis” tab_id=”1684783447897-d707e395-dd7f”][vc_column_text]\n
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    • Please contact us.<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_tta_section][\/vc_tta_accordion][\/vc_column][\/vc_row][vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113623784{padding-top: 60px !important;background-color: #f6f6f6 !important;}”][vc_column][vc_row_inner et_column_align=”align-center” content_placement=”middle”][vc_column_inner animation=”animation bottom-to-top” offset=”vc_col-lg-6″][vc_column_text css=”.vc_custom_1693853737154{padding-right: 10% !important;padding-left: 10% !important;}”]\n

      DNBSEQ\u2122 Sequencing Technology<\/h2>\n

      BGI’s RNA Sequencing services are typically executed with proprietary DNBSEQ\u2122 sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ\u2122 offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ\u2122 platforms.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner et_max_width=”” et_column_align=”align-center” content_placement=”middle”][vc_column_inner animation=”animation right-to-left” offset=”vc_col-lg-6″]\t

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      \n\t\t\t\t\t\"\"\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t<\/div>\n [\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

      [vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}”][vc_column css=”.vc_custom_1684760689814{background-color: #f6f6f6 !important;}”][vc_row_inner][vc_column_inner animation=”animation bottom-to-top”][vc_column_text css=”.vc_custom_1736240293457{margin-bottom: 0px !important;}”]Low-input RNA seq assesses the transcriptome in challenging sample types where there is a small amount of cells, tissue, or RNA available.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113590633{padding-top: 60px !important;background-color: #f6f6f6 !important;}”][vc_column][vc_row_inner et_column_align=”align-center” content_placement=”middle”][vc_column_inner width=”1\/2″ animation=”animation…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4937","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/4937","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4937"}],"version-history":[{"count":6,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/4937\/revisions"}],"predecessor-version":[{"id":4945,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/4937\/revisions\/4945"}],"wp:attachment":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4937"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}