{"id":4108,"date":"2023-05-25T20:18:38","date_gmt":"2023-05-25T20:18:38","guid":{"rendered":"http:\/\/www.geneethic.com.tr\/?page_id=4108"},"modified":"2024-12-04T10:39:16","modified_gmt":"2024-12-04T10:39:16","slug":"intact-mass","status":"publish","type":"page","link":"https:\/\/www.geneethic.com.tr\/?page_id=4108","title":{"rendered":"Proteome Profiling"},"content":{"rendered":"[vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column css=&#8221;.vc_custom_1684760689814{background-color: #f6f6f6 !important;}&#8221;][vc_row_inner][vc_column_inner animation=&#8221;animation bottom-to-top&#8221;]\t<div id=\"et-image-458\" class=\"et-image aligncenter full   et_image_link\">\n\t\t<div class=\"et-image-inner animation bottom-to-top\">\n\t\t\t\t\t\t\t<div class=\"et-image-thumb \">\n\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/m1-300x300.png\" class=\"attachment-medium\" alt=\"\" srcset=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/m1-300x300.png 300w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/m1-150x150.png 150w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/m1.png 500w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t<\/div>\n  [vc_column_text css=&#8221;.vc_custom_1730458928045{margin-bottom: 0px !important;}&#8221;]\n<h3 class=\"2xl:text-4xl xl:text-3xl lg:text-2xl md:text-2xl text-3xl leading-relaxed sm:font-normal font-semibold text-black sm:mb-10 mb-6 mt-0 text-pre-wrap\">Service Description<\/h3>\n<div class=\"text-base text-blank mb-7 text-pre-wrap !leading-loose\">Proteome profiling analysis refers to the analysis of an entire proteome from complex samples like complete cells, tissues and body fluids. It is most often used to identify as many peptides and proteins as possible. Proteome profiling analysis based on mass spectrometry (MS) can provide reference information for high-throughput quantitative proteomics and protein modification analysis.<\/div>\n<div><\/div>\n<div>\n<h3 class=\"2xl:text-4xl xl:text-3xl lg:text-2xl md:text-2xl text-3xl leading-relaxed sm:font-normal font-semibold text-black sm:mb-10 mb-6 mt-0 text-pre-wrap\">Service Specification<\/h3>\n<ul>\n<li class=\"h-full flex sm:flex-row flex-col sm:items-start items-center py-5 sm:pr-2 sm:pl-0 pr-5 pl-5 odd:bg-[#f3f3f3]\">\n<div class=\"flex justify-center items-center w-[160px] flex-shrink-0 relative sm:mb-0 mb-10 \"><img decoding=\"async\" class=\"max-w-[120px]\" src=\"https:\/\/cdn.it.bgi.com\/source\/sample-preparation-services_17.png\" alt=\"\" \/><\/div>\n<div class=\"flex-1\">\n<h4 class=\"text-lg text-[#3ba792] mb-4\">Sample Preparation and Services<\/h4>\n<div>\n<div class=\"style_seve_content__QlWY2\">\n<ul>\n<li>Digestion performed using sequencing-grade trypsin<\/li>\n<li>Off-line sample fractionation using HPLC technology<\/li>\n<li>Each fraction analyzed using nano-flow LC-MS\/MS<\/li>\n<li>Peptide Spectral Match (PSM) data utilized for constructing proteomic profile<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/li>\n<li class=\"h-full flex sm:flex-row flex-col sm:items-start items-center py-5 sm:pr-2 sm:pl-0 pr-5 pl-5 odd:bg-[#f3f3f3]\">\n<div class=\"flex justify-center items-center w-[160px] flex-shrink-0 relative sm:mb-0 mb-10 \"><img decoding=\"async\" class=\"max-w-[120px]\" src=\"https:\/\/cdn.it.bgi.com\/source\/sequencing-qulaity-standard_11.png\" alt=\"\" \/><\/div>\n<div class=\"flex-1\">\n<h4 class=\"text-lg text-[#3ba792] mb-4\">Quality Standard<\/h4>\n<div>\n<div class=\"style_seve_content__QlWY2\">\n<ul>\n<li>Summary includes all methods and data analysis<\/li>\n<li>Reports provided in Excel or PDF format, RAW files available upon request<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/li>\n<li class=\"h-full flex sm:flex-row flex-col sm:items-start items-center py-5 sm:pr-2 sm:pl-0 pr-5 pl-5 odd:bg-[#f3f3f3]\">\n<div class=\"flex justify-center items-center w-[160px] flex-shrink-0 relative sm:mb-0 mb-10 \"><img decoding=\"async\" class=\"max-w-[120px]\" src=\"https:\/\/cdn.it.bgi.com\/source\/turn-around-time.png\" alt=\"\" \/><\/div>\n<div class=\"flex-1\">\n<h4 class=\"text-lg text-[#3ba792] mb-4\">Turn Around Time<\/h4>\n<div>\n<div class=\"style_seve_content__QlWY2\">\n<ul>\n<li>Typical 4-5 weeks from sample QC acceptance to data report delivery<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/li>\n<\/ul>\n<\/div>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113590633{padding-top: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column][vc_row_inner et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner width=&#8221;1\/2&#8243; animation=&#8221;animation bottom-to-top&#8221; offset=&#8221;vc_col-lg-6&#8243;][vc_column_text css=&#8221;.vc_custom_1733308758042{margin-bottom: 0px !important;}&#8221;]\n<h3 class=\"2xl:text-4xl xl:text-3xl lg:text-2xl md:text-2xl text-3xl leading-relaxed sm:font-normal font-semibold text-black sm:mb-10 mb-6 mt-0 text-pre-wrap\">Proteome Profiling\u00a0Project Workflow<\/h3>\n<div class=\"text-base text-blank mb-7 text-pre-wrap !leading-loose\">Proteome profiling is a cost effective, high value solution for monitoring hundreds-to-thousands of proteins simultaneously. We perform sample digestion using sequencing-grade trypsin or alternative proteolytic method, and then trypsin-digested peptide samples are analyzed using nano-flow LC-MS\/MS. The spectral counting method provides information on protein relative abundances. Besides, our HPLC sample fractionation systems are highly recommended for complex sample types to reduce sample complexity and maximize protein sequence coverage and detection dynamic range.<\/div>\n[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243; animation=&#8221;animation bottom-to-top&#8221;][vc_column_text css=&#8221;.vc_custom_1730458891661{margin-bottom: 0px !important;}&#8221;]\n<p style=\"text-align: center;\">SAMPLE PREPARATION<\/p>\n<p style=\"text-align: center;\">LC FRACTIONATION<\/p>\n<p style=\"text-align: center;\">LC-MS\/MS ANALYSIS<\/p>\n<p style=\"text-align: center;\">DATA ANALYSIS<\/p>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner]\t\t<div id=\"et-button-6a9e926d127ea\" class=\"et_btn_align_left animation left-to-right\">\n\t\t\t<a href=\"http:\/\/www.geneethic.com.tr\/?page_id=2221\" class=\" et_btn button et_btn_sm solid color-  arrow-enabled \"  role=\"button\" title=\"Contact_Geneethic\" ><span>Need a quotation?<\/span><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\"\r\n\t width=\"30px\" height=\"18px\" viewBox=\"0 0 30 18\" enable-background=\"new 0 0 30 18\" xml:space=\"preserve\">\r\n<path class=\"handle\" d=\"M20.305,16.212c-0.407,0.409-0.407,1.071,0,1.479s1.068,0.408,1.476,0l7.914-7.952c0.408-0.409,0.408-1.071,0-1.481\r\n\tl-7.914-7.952c-0.407-0.409-1.068-0.409-1.476,0s-0.407,1.071,0,1.48l7.185,7.221L20.305,16.212z\"\/>\r\n<path class=\"bar\" fill-rule=\"evenodd\" clip-rule=\"evenodd\" d=\"M1,8h28.001c0.551,0,1,0.448,1,1c0,0.553-0.449,1-1,1H1c-0.553,0-1-0.447-1-1\r\n\tC0,8.448,0.447,8,1,8z\"\/>\r\n<\/svg>\r\n<\/a>\n\t\t<\/div>\n\t\t\n\t[vc_row_inner et_max_width=&#8221;&#8221; et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner offset=&#8221;vc_col-lg-6&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_column_align=&#8221;align-center&#8221; css=&#8221;.vc_custom_1579113617984{padding-top: 60px !important;padding-bottom: 60px !important;}&#8221;][vc_column width=&#8221;1\/2&#8243; animation=&#8221;animation bottom-to-top&#8221;][vc_tta_accordion active_section=&#8221;1&#8243;][vc_tta_section title=&#8221;Specifications&#8221; tab_id=&#8221;1557543124640-60cb9bb3-2cc8&#8243;][vc_column_text]\n<ul>\n<li>Digestion performed using sequencing-grade trypsin<\/li>\n<li>Off-line sample fractionation using HPLC technology<\/li>\n<li>Each fraction analyzed using nano-flow LC-MS\/MS<\/li>\n<li>Peptide Spectral Match (PSM) data utilized for constructing proteomic profile<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Sample Requirements&#8221; tab_id=&#8221;1557543124710-e0287025-a0eb&#8221;][vc_column_text]\n<table>\n<thead>\n<tr>\n<th class=\"style_table_cell__2wtB2\">SAMPLE TYPE<\/th>\n<th class=\"style_table_cell__2wtB2\">AMOUNT AND CONCENTRATION<\/th>\n<th class=\"style_table_cell__2wtB2\">MINIMUM SAMPLE VOLUME<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td class=\"style_table_cell__2wtB2\">Protein sample in liquid solution or in gel band\/spot<\/td>\n<td class=\"style_table_cell__2wtB2\">Recommended 100 \u00b5g for each enzyme: 2 \u00b5g\/\u00b5L<\/td>\n<td class=\"style_table_cell__2wtB2\">100 \u00b5L for each enzyme<\/td>\n<\/tr>\n<tr>\n<td class=\"style_table_cell__2wtB2\">Protein sample in liquid solution or in gel band\/spot<\/td>\n<td class=\"style_table_cell__2wtB2\">Minimum Required 10 \u00b5g for each enzyme: 1 \u00b5g\/\u00b5L<\/td>\n<td class=\"style_table_cell__2wtB2\">10 \u00b5L for each enzyme<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Advanced Bioinformatics Analysis&#8221; tab_id=&#8221;1684783447897-d707e395-dd7f&#8221;][vc_column_text]\n<ul>\n<li>Data analysis and validation performed with Mascot<\/li>\n<li>GO (Gene ontology) category analysis<\/li>\n<li>COG (Cluster of Orthologous Groups of proteins) category analysis<\/li>\n<li>Pathway analysis<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_tta_section][\/vc_tta_accordion][vc_column_text animation=&#8221;animation bottom-to-top&#8221;]\n<p style=\"text-align: center;\"><strong><a href=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/Proteome-Profiling-Service-Overview.pdf\" target=\"_blank\" rel=\"noopener\">Proteome Profiling Service Overview<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4369 size-thumbnail\" src=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-150x150.webp\" alt=\"\" width=\"150\" height=\"150\" srcset=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-150x150.webp 150w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-300x300.webp 300w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-1024x1024.webp 1024w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-768x768.webp 768w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280.webp 1280w\" sizes=\"auto, (max-width: 150px) 100vw, 150px\" \/><\/a><br \/>\n<\/strong>[\/vc_column_text][\/vc_column][\/vc_row][vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113623784{padding-top: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column][vc_row_inner et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner animation=&#8221;animation bottom-to-top&#8221; offset=&#8221;vc_col-lg-6&#8243;][vc_column_text css=&#8221;.vc_custom_1693853529047{padding-right: 10% !important;padding-left: 10% !important;}&#8221;]\n<h2 style=\"text-align: center;\">Unique DNBSEQ\u2122 Sequencing Technology<\/h2>\n<p>BGI&#8217;s RNA Sequencing services are typically executed with proprietary DNBSEQ\u2122 sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ\u2122 offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ\u2122 platforms.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner et_max_width=&#8221;&#8221; et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner animation=&#8221;animation right-to-left&#8221; offset=&#8221;vc_col-lg-6&#8243;]\t<div id=\"et-image-626\" class=\"et-image     et_image_link\">\n\t\t<div class=\"et-image-inner animation bottom-to-top\">\n\t\t\t\t\t\t\t<div class=\"et-image-thumb \">\n\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1522\" height=\"582\" src=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede.png\" class=\"attachment-full\" alt=\"\" srcset=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede.png 1522w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-300x115.png 300w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-1024x392.png 1024w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-768x294.png 768w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-150x57.png 150w\" sizes=\"auto, (max-width: 1522px) 100vw, 1522px\" \/>\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t<\/div>\n  [\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"<p>[vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column css=&#8221;.vc_custom_1684760689814{background-color: #f6f6f6 !important;}&#8221;][vc_row_inner][vc_column_inner animation=&#8221;animation bottom-to-top&#8221;][vc_column_text css=&#8221;.vc_custom_1730458928045{margin-bottom: 0px !important;}&#8221;] Service Description Proteome profiling analysis refers to the analysis of an entire proteome from complex samples like complete cells, tissues and body fluids. It is most often used to identify as many&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4108","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/4108","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4108"}],"version-history":[{"count":7,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/4108\/revisions"}],"predecessor-version":[{"id":4926,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/4108\/revisions\/4926"}],"wp:attachment":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4108"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}