{"id":4090,"date":"2023-05-25T20:09:09","date_gmt":"2023-05-25T20:09:09","guid":{"rendered":"http:\/\/www.geneethic.com.tr\/?page_id=4090"},"modified":"2024-11-12T11:48:59","modified_gmt":"2024-11-12T11:48:59","slug":"single-cell-rna-seq","status":"publish","type":"page","link":"https:\/\/www.geneethic.com.tr\/?page_id=4090","title":{"rendered":"Isobaric Label"},"content":{"rendered":"[vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}”][vc_column css=”.vc_custom_1684760689814{background-color: #f6f6f6 !important;}”][vc_row_inner][vc_column_inner animation=”animation bottom-to-top”]\t
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Service Description<\/h3>\n
We have developed innovative solutions for optimizing the process of accurately quantifying proteins in complex biological matrices and other complex mixtures.<\/div>\n
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Mass Spectrometry Service Specification<\/h3>\n
Isobaric Label Quantitative Proteomics services are performed using nano-flow liquid chromatography and cutting-edge mass spectrometer (Q Exactive HF\/HF-X).<\/div>\n
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Sample Preparation and Services<\/h4>\n<\/div>\n<\/div>\n

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The QC of enzymatic hydrolysis and labeling steps are strictly controlled by LC-MS DDA analysis<\/p>\n

1h\/fraction; 20 fractions for eukaryotes;10 fractions for prokaryotes<\/p>\n

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Quality Standard<\/h4>\n
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Summary includes all methods and data analysis<\/p>\n

Reports provided in Excel or PDF format, RAW files available upon request<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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Turn Around Time<\/h4>\n

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Typical 4-5 weeks from sample QC acceptance to data report delivery<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113590633{padding-top: 60px !important;background-color: #f6f6f6 !important;}”][vc_column][vc_row_inner et_column_align=”align-center” content_placement=”middle”][vc_column_inner width=”1\/2″ animation=”animation bottom-to-top” offset=”vc_col-lg-6″][vc_column_text css=”.vc_custom_1730468514462{margin-bottom: 0px !important;}”]\n

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Isobaric Label TMT Quantitation Proteomics<\/h3>\n
Isobaric Label services incorporate sample multiplexing to provide deep proteome coverage and highly precise quantitation of small or medium-sized sample sets. Samples are prepared and labeled separately, then all samples are pooled and analyzed by nano-flow LC-MS\/MS1.<\/div>\n<\/div>\n<\/div>\n
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Project Workflow<\/h3>\n
BGI Genomics provides Isobaric Label Quantitative Proteomics service using iTRAQ (Isobaric Tags for Relative and Absolute Quantitation), IBT (Isobaric Tags for Quantitation) and TMT (Tandem Mass Tags for Quantitation) technologies developed by AB SCIEX, BGI and Thermo Scientific, respectively.<\/div>\n