{"id":4088,"date":"2023-05-25T20:08:57","date_gmt":"2023-05-25T20:08:57","guid":{"rendered":"http:\/\/www.geneethic.com.tr\/?page_id=4088"},"modified":"2024-11-12T11:49:29","modified_gmt":"2024-11-12T11:49:29","slug":"targeted-proteomics","status":"publish","type":"page","link":"https:\/\/www.geneethic.com.tr\/?page_id=4088","title":{"rendered":"Label-free DDA Proteomics"},"content":{"rendered":"[vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column css=&#8221;.vc_custom_1684760689814{background-color: #f6f6f6 !important;}&#8221;][vc_row_inner][vc_column_inner animation=&#8221;animation bottom-to-top&#8221;]\t<div id=\"et-image-311\" class=\"et-image aligncenter    et_image_link\">\n\t\t<div class=\"et-image-inner animation bottom-to-top\">\n\t\t\t\t\t\t\t<div class=\"et-image-thumb \">\n\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/m4-300x300.png\" class=\"attachment-medium\" alt=\"\" srcset=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/m4-300x300.png 300w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/m4-150x150.png 150w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/m4.png 500w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t<\/div>\n  [vc_column_text css=&#8221;.vc_custom_1730488967738{margin-bottom: 0px !important;}&#8221;]\n<h4 class=\"2xl:text-4xl xl:text-3xl lg:text-2xl md:text-2xl text-3xl leading-relaxed sm:font-normal font-semibold text-black sm:mb-10 mb-6 mt-0 text-pre-wrap\">Service Description<\/h4>\n<p class=\"2xl:text-4xl xl:text-3xl lg:text-2xl md:text-2xl text-3xl leading-relaxed sm:font-normal font-semibold text-black sm:mb-10 mb-6 mt-0 text-pre-wrap\">\nQuantitative proteomics refers to the identification and quantification analysis of proteins in two or more samples to obtain the differential expression and function information of relevant proteins. It plays an important research role in the prevention, diagnosis, prognosis and efficacy monitoring of diseases, and can be used to help develop new therapeutic drugs.<\/p>\n<p class=\"2xl:text-4xl xl:text-3xl lg:text-2xl md:text-2xl text-3xl leading-relaxed sm:font-normal font-semibold text-black sm:mb-10 mb-6 mt-0 text-pre-wrap\">Liquid chromatography &#8211; tandem mass spectrometry (LC-MS\/MS) is currently the best method for unbiased, high-throughput proteomics analysis. In contrast to label-dependent quantitative proteomics such as the iTRAQ method, label-free data-dependent acquisition (DDA) proteomics doesn\u2019t need to use expensive a stable isotope for labeling of proteins. This allows accurate protein quantification of samples without additional error-prone in vitro labelling reactions and enables fast proteome identification and quantification.<br \/>\nIn a typical DDA analysis, the mass spectrometer generates a full-scan mass spectra to determine the molecular weights of peptides and then acquires MS\/MS spectra on the N most intense peptide ions. Hundreds of MS\/MS spectra can be generated in a single run and downstream data analysis tools are applied for peptide identification and quantification. Since the peptides selected for fragmentation are usually the most abundant peaks in the survey scan, it is easy to cause the loss of low abundance peptides.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113590633{padding-top: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column][vc_row_inner et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner width=&#8221;1\/2&#8243; animation=&#8221;animation bottom-to-top&#8221; offset=&#8221;vc_col-lg-6&#8243;][vc_column_text css=&#8221;.vc_custom_1730489075095{margin-bottom: 0px !important;}&#8221;]\n<div class=\"style_section__3nkqm\">\n<div class=\"style_richtext__23EN8 style_section_wrapper__39N5M style_section_richtext__2sE4K \">\n<h3 class=\"2xl:text-4xl xl:text-3xl lg:text-2xl md:text-2xl text-3xl leading-relaxed sm:font-normal font-semibold text-black sm:mb-10 mb-6 mt-0 text-pre-wrap\">Label Free DDA Analysis Workflow<\/h3>\n<ul>\n<li>Protein extraction<\/li>\n<li>Protein digestion<\/li>\n<li>HPLC fractionation<\/li>\n<li>LC-MS\/MS(DDA)<\/li>\n<li>Data analysis<\/li>\n<\/ul>\n<\/div>\n<\/div>\n[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243; animation=&#8221;animation bottom-to-top&#8221;][vc_column_text css=&#8221;.vc_custom_1730489018610{margin-bottom: 0px !important;}&#8221;]\n<div class=\"md:px-[13%] px-[5%] mb-14\">\n<h3 class=\"2xl:text-4xl xl:text-3xl lg:text-2xl md:text-2xl text-3xl leading-relaxed sm:font-normal font-semibold text-black sm:mb-10 mb-6 mt-0 text-pre-wrap\" style=\"text-align: center;\">Research Applications<\/h3>\n<div class=\"text-base text-blank mb-7 text-pre-wrap !leading-loose\" style=\"text-align: left;\">Disease biomarker research<br \/>\nDrug efficacy evaluation<br \/>\nSpecial behavior mechanism and food\/medicinal value research<br \/>\nPlant growth and development research<br \/>\nPlant disease resistance and insect resistance research<br \/>\nMicrobial stress and adversity physiology research<\/div>\n<\/div>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner]\t\t<div id=\"et-button-6a10a0dbdffd2\" class=\"et_btn_align_left animation left-to-right\">\n\t\t\t<a href=\"http:\/\/www.geneethic.com.tr\/?page_id=2221\" class=\" et_btn button et_btn_sm solid color-  arrow-enabled \"  role=\"button\" title=\"Contact_Geneethic\" ><span>Need a quotation?<\/span><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\"\r\n\t width=\"30px\" height=\"18px\" viewBox=\"0 0 30 18\" enable-background=\"new 0 0 30 18\" xml:space=\"preserve\">\r\n<path class=\"handle\" d=\"M20.305,16.212c-0.407,0.409-0.407,1.071,0,1.479s1.068,0.408,1.476,0l7.914-7.952c0.408-0.409,0.408-1.071,0-1.481\r\n\tl-7.914-7.952c-0.407-0.409-1.068-0.409-1.476,0s-0.407,1.071,0,1.48l7.185,7.221L20.305,16.212z\"\/>\r\n<path class=\"bar\" fill-rule=\"evenodd\" clip-rule=\"evenodd\" d=\"M1,8h28.001c0.551,0,1,0.448,1,1c0,0.553-0.449,1-1,1H1c-0.553,0-1-0.447-1-1\r\n\tC0,8.448,0.447,8,1,8z\"\/>\r\n<\/svg>\r\n<\/a>\n\t\t<\/div>\n\t\t\n\t[vc_row_inner et_max_width=&#8221;&#8221; et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner offset=&#8221;vc_col-lg-6&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_column_align=&#8221;align-center&#8221; css=&#8221;.vc_custom_1579113617984{padding-top: 60px !important;padding-bottom: 60px !important;}&#8221;][vc_column width=&#8221;1\/2&#8243; animation=&#8221;animation bottom-to-top&#8221;][vc_tta_accordion active_section=&#8221;1&#8243;][vc_tta_section title=&#8221;Specifications&#8221; tab_id=&#8221;1557543124640-60cb9bb3-2cc8&#8243;][vc_column_text]\n<ul>\n<li>The QC of enzymatic hydrolysis is strictly controlled by LC-MS<\/li>\n<li>2h for DDA analysis<\/li>\n<li>Summary includes all methods and data analysis<\/li>\n<li>Reports provided in Excel or PDF format, RAW files available upon request<\/li>\n<li>Typical 3-4 weeks from sample QC acceptance to data report delivery<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Sample Requirements&#8221; tab_id=&#8221;1557543124710-e0287025-a0eb&#8221;][vc_column_text]Please see the catalogue[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Data Analysis&#8221; tab_id=&#8221;1684783447897-d707e395-dd7f&#8221;][vc_column_text]\n<ul>\n<li>Data analysis and validation performed with Mascot<\/li>\n<li>Protein identification and quantification list<\/li>\n<li>Differential proteins data statistics and volcano plot<\/li>\n<li>Principal component analysis (PCA) and cluster analysis<\/li>\n<li>Protein GO\/COG\/KOG\/Pathway annotation<\/li>\n<li>GO\/COG\/KOG enrichment analysis of differential proteins<\/li>\n<li>Protein-protein interaction analysis<\/li>\n<li>Protein subcellular localization analysis<\/li>\n<li>Time series analysis<\/li>\n<li>Repeatability analysis<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_tta_section][\/vc_tta_accordion][vc_row_inner][vc_column_inner][vc_column_text animation=&#8221;animation bottom-to-top&#8221;]\n<div class=\"md:px-[13%] px-[5%] pt-40 pb-10 2xl:h-[298px] sm:min-h-min min-h-[15rem] bg-no-repeat bg-cover bg-center bg-gradient\">\n<div class=\"md:px-[13%] px-[5%] pt-40 pb-10 2xl:h-[298px] sm:min-h-min min-h-[15rem] bg-no-repeat bg-cover bg-center bg-gradient\">\n<p class=\"text-white text-pre-wrap 2xl:text-6xl xl:text-5xl lg:text-4xl md:text-3xl text-4xl\" style=\"text-align: center;\"><strong><a href=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/Label-Free-DDA-Proteomics-Service-Overview202102.pdf\" target=\"_blank\" rel=\"noopener\">Label-free DDA Proteomics<\/a><\/strong><\/p>\n<\/div>\n<\/div>\n<p style=\"text-align: center;\"><strong><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4369 aligncenter\" src=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-300x300.webp\" alt=\"\" width=\"75\" height=\"75\" srcset=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-300x300.webp 300w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-1024x1024.webp 1024w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-150x150.webp 150w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-768x768.webp 768w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280.webp 1280w\" sizes=\"auto, (max-width: 75px) 100vw, 75px\" \/><\/strong><\/p>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113623784{padding-top: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column][vc_row_inner et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner animation=&#8221;animation bottom-to-top&#8221; offset=&#8221;vc_col-lg-6&#8243;][vc_column_text css=&#8221;.vc_custom_1699874697862{padding-right: 10% !important;padding-left: 10% !important;}&#8221;]\n<h2 style=\"text-align: center;\">Unique DNBSEQ\u2122 Sequencing Technology<\/h2>\n<p>BGI&#8217;s Single Cell Sequencing services are typically executed with proprietary DNBSEQ\u2122 sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ\u2122 can offer advantages in terms of lower amplification error rates and much lower duplication rates for some sequencing applications. In addition, studies have shown the lower index hopping rate in DNBSEQ\u2122 platforms.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner et_max_width=&#8221;&#8221; et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner animation=&#8221;animation right-to-left&#8221; offset=&#8221;vc_col-lg-6&#8243;]\t<div id=\"et-image-56\" class=\"et-image     et_image_link\">\n\t\t<div class=\"et-image-inner animation bottom-to-top\">\n\t\t\t\t\t\t\t<div class=\"et-image-thumb \">\n\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1522\" height=\"582\" src=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede.png\" class=\"attachment-full\" alt=\"\" srcset=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede.png 1522w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-300x115.png 300w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-1024x392.png 1024w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-768x294.png 768w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-150x57.png 150w\" sizes=\"auto, (max-width: 1522px) 100vw, 1522px\" \/>\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t<\/div>\n  [\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"<p>[vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column css=&#8221;.vc_custom_1684760689814{background-color: #f6f6f6 !important;}&#8221;][vc_row_inner][vc_column_inner animation=&#8221;animation bottom-to-top&#8221;][vc_column_text css=&#8221;.vc_custom_1730488967738{margin-bottom: 0px !important;}&#8221;] Service Description Quantitative proteomics refers to the identification and quantification analysis of proteins in two or more samples to obtain the differential expression and function information of relevant proteins. It plays an&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4088","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/4088","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4088"}],"version-history":[{"count":8,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/4088\/revisions"}],"predecessor-version":[{"id":4882,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/4088\/revisions\/4882"}],"wp:attachment":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4088"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}