{"id":4086,"date":"2023-05-25T20:08:45","date_gmt":"2023-05-25T20:08:45","guid":{"rendered":"http:\/\/www.geneethic.com.tr\/?page_id=4086"},"modified":"2024-11-12T11:54:55","modified_gmt":"2024-11-12T11:54:55","slug":"label-free-dda-proteomics","status":"publish","type":"page","link":"https:\/\/www.geneethic.com.tr\/?page_id=4086","title":{"rendered":"Nanoproteomics"},"content":{"rendered":"[vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}”][vc_column css=”.vc_custom_1684760689814{background-color: #f6f6f6 !important;}”][vc_row_inner][vc_column_inner animation=”animation bottom-to-top”]\t
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Nanoproteomics Service Overview<\/h4>\n

To date, most proteomics studies have been based on proteins obtained from the homogenization of large volumes of cells or tissues. However, with the advancement of medical research, there is a growing need for the analysis of highly specialized specimens within specific applications and research areas such as trace specimens identified by forensic experts, or intra-tumor heterogeneity research.
\nNanoproteomics refers to quantitative proteomics analysis of small cell populations (typically < 5,000 cells) by the combination of in-situ cleavage and DDA label free technology, and offers possibilities for analysis unavailable with conventional protein extraction and mass spectrometry.
\nBGI has extensive experience in the field of nanoproteomics spanning cellular heterogeneity research across rare cell populations, hard-to-obtain clinical specimens, and pathological tissues.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113590633{padding-top: 60px !important;background-color: #f6f6f6 !important;}”][vc_column][vc_row_inner et_column_align=”align-center” content_placement=”middle”][vc_column_inner width=”1\/2″ animation=”animation bottom-to-top” offset=”vc_col-lg-6″][vc_column_text css=”.vc_custom_1730485401403{margin-bottom: 0px !important;}”]\n

Mass Spectrometry Service Specification<\/h4>\n

Nanoproteomics services are performed using nano-flow liquid chromatography and cutting-edge Orbitrap Fusion Lumos mass spectrometer.<\/p>\n

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Sample Preparation and Services<\/h4>\n
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The QC of enzymatic hydrolysis is strictly controlled by LC-MS<\/p>\n

DDA-Spectral Library generated using HPLC technology and sample fractionation<\/p>\n

2h\/fraction; 2h for DDA analysis<\/p>\n

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Quality Standard<\/h4>\n
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Summary includes all methods and data analysis<\/p>\n

Reports provided in Excel or PDF format, RAW files available upon request<\/p>\n

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Turn Around Time<\/h4>\n
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Typical 5-6 weeks from sample QC acceptance to data report delivery<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″ animation=”animation bottom-to-top”][vc_column_text css=”.vc_custom_1730485407521{margin-bottom: 0px !important;}”]\n

Workflow<\/h4>\n

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