{"id":4084,"date":"2023-05-25T20:08:29","date_gmt":"2023-05-25T20:08:29","guid":{"rendered":"http:\/\/www.geneethic.com.tr\/?page_id=4084"},"modified":"2024-11-01T09:44:18","modified_gmt":"2024-11-01T09:44:18","slug":"isobaric-label","status":"publish","type":"page","link":"https:\/\/www.geneethic.com.tr\/?page_id=4084","title":{"rendered":"Protein Identification"},"content":{"rendered":"[vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column css=&#8221;.vc_custom_1684760689814{background-color: #f6f6f6 !important;}&#8221;][vc_row_inner][vc_column_inner animation=&#8221;animation bottom-to-top&#8221;]\t<div id=\"et-image-608\" class=\"et-image aligncenter full   et_image_link\">\n\t\t<div class=\"et-image-inner animation bottom-to-top\">\n\t\t\t\t\t\t\t<div class=\"et-image-thumb \">\n\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"360\" height=\"360\" src=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/pngtree-molecule-vector-illustration-design-biotechnology-protein-molecules-vector-png-image_38451540.png\" class=\"attachment-large\" alt=\"\" srcset=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/pngtree-molecule-vector-illustration-design-biotechnology-protein-molecules-vector-png-image_38451540.png 360w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/pngtree-molecule-vector-illustration-design-biotechnology-protein-molecules-vector-png-image_38451540-300x300.png 300w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/pngtree-molecule-vector-illustration-design-biotechnology-protein-molecules-vector-png-image_38451540-150x150.png 150w\" sizes=\"auto, (max-width: 360px) 100vw, 360px\" \/>\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t<\/div>\n  [vc_column_text css=&#8221;.vc_custom_1730454258019{margin-bottom: 0px !important;}&#8221;]\n<h3 class=\"2xl:text-4xl xl:text-3xl lg:text-2xl md:text-2xl text-3xl leading-relaxed sm:font-normal font-semibold text-black sm:mb-10 mb-6 mt-0 text-pre-wrap\">Service Description<\/h3>\n<div class=\"text-base text-blank mb-7 text-pre-wrap !leading-loose\">Modern liquid chromatography and mass spectrometry technology provides a means to routinely perform non-targeted protein identification. BGI can help simplify the challenge of protein identification for your project.<\/div>\n<h3><\/h3>\n<h3><\/h3>\n<h3 class=\"2xl:text-4xl xl:text-3xl lg:text-2xl md:text-2xl text-3xl leading-relaxed sm:font-normal font-semibold text-black sm:mb-10 mb-6 mt-0 text-pre-wrap\">Mass Spectrometry Service Specification<\/h3>\n<div class=\"text-base text-blank mb-7 text-pre-wrap !leading-loose\">Protein Identification services are performed using nano-flow liquid chromatography and high resolution Orbitrap mass spectrometry.<\/div>\n<ul>\n<li class=\"h-full flex sm:flex-row flex-col sm:items-start items-center py-5 sm:pr-2 sm:pl-0 pr-5 pl-5 odd:bg-[#f3f3f3]\">\n<div class=\"flex justify-center items-center w-[160px] flex-shrink-0 relative sm:mb-0 mb-10 \"><img decoding=\"async\" class=\"max-w-[120px]\" src=\"https:\/\/cdn.it.bgi.com\/source\/sample-preparation-services_8.png\" alt=\"\" \/><\/div>\n<div class=\"flex-1\">\n<h4 class=\"text-lg text-[#3ba792] mb-4\">Sample Preparation and Services<\/h4>\n<div>\n<div class=\"style_seve_content__QlWY2\">\n<ul>\n<li>Digestion performed using sequencing-grade trypsin<\/li>\n<li>60 min nano-flow LC-MS\/MS using a Q Exactive HF-X Orbitrap mass spectrometer<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/li>\n<li class=\"h-full flex sm:flex-row flex-col sm:items-start items-center py-5 sm:pr-2 sm:pl-0 pr-5 pl-5 odd:bg-[#f3f3f3]\">\n<div class=\"flex justify-center items-center w-[160px] flex-shrink-0 relative sm:mb-0 mb-10 \"><img decoding=\"async\" class=\"max-w-[120px]\" src=\"https:\/\/cdn.it.bgi.com\/source\/sequencing-qulaity-standard_2.png\" alt=\"\" \/><\/div>\n<div class=\"flex-1\">\n<h4 class=\"text-lg text-[#3ba792] mb-4\">Mass Spec Services Quality Standard<\/h4>\n<div>\n<div class=\"style_seve_content__QlWY2\">\n<ul>\n<li>Summary including all methods and data analysis<\/li>\n<li>Reports provided in Excel or PDF format, RAW files available upon request<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/li>\n<li class=\"h-full flex sm:flex-row flex-col sm:items-start items-center py-5 sm:pr-2 sm:pl-0 pr-5 pl-5 odd:bg-[#f3f3f3]\">\n<div class=\"flex justify-center items-center w-[160px] flex-shrink-0 relative sm:mb-0 mb-10 \"><img decoding=\"async\" class=\"max-w-[120px]\" src=\"https:\/\/cdn.it.bgi.com\/source\/turn-around-time_24.png\" alt=\"\" \/><\/div>\n<div class=\"flex-1\">\n<h4 class=\"text-lg text-[#3ba792] mb-4\">Turn Around Time<\/h4>\n<div>\n<div class=\"style_seve_content__QlWY2\">\n<ul>\n<li>Typical 3-4 weeks from sample QC acceptance to data report delivery<\/li>\n<li>Prime Expedited Services with 1 week Turn Around time is available<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113590633{padding-top: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column][vc_row_inner et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner width=&#8221;1\/2&#8243; animation=&#8221;animation bottom-to-top&#8221; offset=&#8221;vc_col-lg-6&#8243;][vc_column_text css=&#8221;.vc_custom_1730453187584{margin-bottom: 0px !important;}&#8221;]\n<h3 class=\"2xl:text-4xl xl:text-3xl lg:text-2xl md:text-2xl text-3xl leading-relaxed sm:font-normal font-semibold text-black sm:mb-10 mb-6 mt-0 text-pre-wrap\" style=\"text-align: center;\">In-Gel Workflow<\/h3>\n<p class=\"inline-block text-gradient 2xl:text-2xl text-xl sm:mb-4 mb-6 text-pre-wrap\">In-Gel Identification<\/p>\n<div class=\"text-base text-blank mb-7 text-pre-wrap !leading-loose\">We can identify proteins isolated in gel bands or spots. Our service includes destaining, in-gel digestion, nano-flow LC-MS\/MS, and peptide spectral match data analysis.<\/div>\n[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243; animation=&#8221;animation bottom-to-top&#8221;][vc_column_text css=&#8221;.vc_custom_1730453193933{margin-bottom: 0px !important;}&#8221;]\n<h3 class=\"2xl:text-4xl xl:text-3xl lg:text-2xl md:text-2xl text-3xl leading-relaxed sm:font-normal font-semibold text-black sm:mb-10 mb-6 mt-0 text-pre-wrap\" style=\"text-align: center;\">In-Solution Workflow<\/h3>\n<p class=\"inline-block text-gradient 2xl:text-2xl text-xl sm:mb-4 mb-6 text-pre-wrap\">In-Solution Identification<\/p>\n<div class=\"text-base text-blank mb-7 text-pre-wrap !leading-loose\">Our in-solution protein identification services can be used for applications such as monitoring recombinant protein expression and affinity purification systems.<\/div>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner]\t\t<div id=\"et-button-6a60e4e906fc0\" class=\"et_btn_align_left animation left-to-right\">\n\t\t\t<a href=\"http:\/\/www.geneethic.com.tr\/?page_id=2221\" class=\" et_btn button et_btn_sm solid color-  arrow-enabled \"  role=\"button\" title=\"Contact_Geneethic\" ><span>Need a quotation?<\/span><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\"\r\n\t width=\"30px\" height=\"18px\" viewBox=\"0 0 30 18\" enable-background=\"new 0 0 30 18\" xml:space=\"preserve\">\r\n<path class=\"handle\" d=\"M20.305,16.212c-0.407,0.409-0.407,1.071,0,1.479s1.068,0.408,1.476,0l7.914-7.952c0.408-0.409,0.408-1.071,0-1.481\r\n\tl-7.914-7.952c-0.407-0.409-1.068-0.409-1.476,0s-0.407,1.071,0,1.48l7.185,7.221L20.305,16.212z\"\/>\r\n<path class=\"bar\" fill-rule=\"evenodd\" clip-rule=\"evenodd\" d=\"M1,8h28.001c0.551,0,1,0.448,1,1c0,0.553-0.449,1-1,1H1c-0.553,0-1-0.447-1-1\r\n\tC0,8.448,0.447,8,1,8z\"\/>\r\n<\/svg>\r\n<\/a>\n\t\t<\/div>\n\t\t\n\t[vc_row_inner et_max_width=&#8221;&#8221; et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner offset=&#8221;vc_col-lg-6&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_column_align=&#8221;align-center&#8221; css=&#8221;.vc_custom_1579113617984{padding-top: 60px !important;padding-bottom: 60px !important;}&#8221;][vc_column width=&#8221;1\/2&#8243; animation=&#8221;animation bottom-to-top&#8221;][vc_tta_accordion active_section=&#8221;1&#8243;][vc_tta_section title=&#8221;Specifications&#8221; tab_id=&#8221;1557543124640-60cb9bb3-2cc8&#8243;][vc_column_text]\n<ul>\n<li>\n<div class=\"text-base text-blank mb-7 text-pre-wrap !leading-loose\">We accept protein samples in solution or gel format. For complex in-solution samples we can provide electrophoresis services, including preparative SDS-PAGE and analytical staining.<\/div>\n<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Sample Requirements&#8221; tab_id=&#8221;1557543124710-e0287025-a0eb&#8221;][vc_column_text]\n<table>\n<thead>\n<tr>\n<th class=\"style_table_cell__2wtB2\">Sample type<\/th>\n<th class=\"style_table_cell__2wtB2\">Amount and Concentration<\/th>\n<th class=\"style_table_cell__2wtB2\">Minimum sample volume<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td class=\"style_table_cell__2wtB2\">Protein sample in liquid solution or gel band\/spot<\/td>\n<td class=\"style_table_cell__2wtB2\">Recommended 100 \u00b5g; 2 \u00b5g\/\u00b5L or above<\/td>\n<td class=\"style_table_cell__2wtB2\">10 \u00b5L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Advanced Bioinformatics Analysis&#8221; tab_id=&#8221;1684783447897-d707e395-dd7f&#8221;][vc_column_text]\n<ul>\n<li>Data analysis and validation performed with SEQUEST or Mascot<\/li>\n<li>Top protein identifications, peptide spectral match count, and sequence coverage provided<\/li>\n<li>Up to 30 proteins ranked by peptide spectral match count<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_tta_section][\/vc_tta_accordion][vc_column_text animation=&#8221;animation bottom-to-top&#8221;]\n<p style=\"text-align: center;\"><strong><a href=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2024\/11\/Protein-Identification-Service-Overview.pdf\" target=\"_blank\" rel=\"noopener\">Protein Identification Service Overview<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4369 size-thumbnail\" src=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-150x150.webp\" alt=\"\" width=\"150\" height=\"150\" srcset=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-150x150.webp 150w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-300x300.webp 300w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-1024x1024.webp 1024w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-768x768.webp 768w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280.webp 1280w\" sizes=\"auto, (max-width: 150px) 100vw, 150px\" \/><\/a><br \/>\n<\/strong><\/p>\n<p>&nbsp;[\/vc_column_text][\/vc_column][\/vc_row][vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113623784{padding-top: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column][vc_row_inner et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner animation=&#8221;animation bottom-to-top&#8221; offset=&#8221;vc_col-lg-6&#8243;][vc_column_text css=&#8221;.vc_custom_1693853529047{padding-right: 10% !important;padding-left: 10% !important;}&#8221;]\n<h2 style=\"text-align: center;\">Unique DNBSEQ\u2122 Sequencing Technology<\/h2>\n<p>BGI&#8217;s RNA Sequencing services are typically executed with proprietary DNBSEQ\u2122 sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ\u2122 offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ\u2122 platforms.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner et_max_width=&#8221;&#8221; et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner animation=&#8221;animation right-to-left&#8221; offset=&#8221;vc_col-lg-6&#8243;]\t<div id=\"et-image-268\" class=\"et-image     et_image_link\">\n\t\t<div class=\"et-image-inner animation bottom-to-top\">\n\t\t\t\t\t\t\t<div class=\"et-image-thumb \">\n\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1522\" height=\"582\" src=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede.png\" class=\"attachment-full\" alt=\"\" srcset=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede.png 1522w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-300x115.png 300w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-1024x392.png 1024w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-768x294.png 768w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-150x57.png 150w\" sizes=\"auto, (max-width: 1522px) 100vw, 1522px\" \/>\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t<\/div>\n  [\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"<p>[vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column css=&#8221;.vc_custom_1684760689814{background-color: #f6f6f6 !important;}&#8221;][vc_row_inner][vc_column_inner animation=&#8221;animation bottom-to-top&#8221;][vc_column_text css=&#8221;.vc_custom_1730454258019{margin-bottom: 0px !important;}&#8221;] Service Description Modern liquid chromatography and mass spectrometry technology provides a means to routinely perform non-targeted protein identification. BGI can help simplify the challenge of protein identification for your project. Mass Spectrometry&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-4084","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/4084","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4084"}],"version-history":[{"count":12,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/4084\/revisions"}],"predecessor-version":[{"id":4580,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/4084\/revisions\/4580"}],"wp:attachment":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4084"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}