{"id":3900,"date":"2023-05-24T10:26:33","date_gmt":"2023-05-24T10:26:33","guid":{"rendered":"http:\/\/www.geneethic.com.tr\/?page_id=3900"},"modified":"2024-01-08T09:03:37","modified_gmt":"2024-01-08T09:03:37","slug":"circrna-sequencing","status":"publish","type":"page","link":"https:\/\/www.geneethic.com.tr\/?page_id=3900","title":{"rendered":"circRNA Sequencing"},"content":{"rendered":"[vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}”][vc_column css=”.vc_custom_1684760689814{background-color: #f6f6f6 !important;}”][vc_row_inner][vc_column_inner animation=”animation bottom-to-top”]\t
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circRNAs are a novel class of abundant, stable and ubiquitous RNAs. circRNAs are distinguished from mRNAs in that they lack poly (A) tails and 5\u0374 caps, and are resistant to exonuclease treatment. circRNAs can serve as miRNA or RNA-binding protein \u2018sponges\u2019, sequestering miRNAs and preventing their interactions with target mRNAs, as a result, controlling transcriptional events.<\/p>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113590633{padding-top: 60px !important;background-color: #f6f6f6 !important;}”][vc_column][vc_row_inner et_column_align=”align-center” content_placement=”middle”][vc_column_inner width=”1\/2″ animation=”animation bottom-to-top” offset=”vc_col-lg-6″][vc_column_text css=”.vc_custom_1684924359695{margin-bottom: 0px !important;}”]Circular RNAs (circRNAs) are produced from precursor mRNA (pre-mRNA) back-splicing of thousands of genes in eukaryotes. Although circRNAs are generally expressed at low levels, recent findings have shed new light on their cell type-specific and tissue-specific expression and on the regulation of their biogenesis. Furthermore, the data indicate that circRNAs shape gene expression by titrating microRNAs, regulating transcription and interfering with splicing, thus effectively expanding the diversity and complexity of eukaryotic transcriptomes.<\/p>\n

In addition to the circular RNAs that are formed from thousands of loci in eukaryotes by exon back-splicing circularization, different types of circular RNAs exist, which are produced by distinct mechanisms. First, circular viral RNA genomes are ligated by host cellular enzymes to form 3\u0374,5\u0374-phosphodiester bonds or 2\u0374,5\u0374-phosphodiester bonds.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″ animation=”animation bottom-to-top”][vc_column_text css=”.vc_custom_1684924353815{margin-bottom: 0px !important;}”]Second, circular RNA intermediates can be generated during rRNA processing and permuted tRNA biogenesis in Archaea and algae. Third, a number of housekeeping non-coding RNAs, including small nucleolar RNAs and the ribozyme RNase P, were also identified in circular forms in Archaea. Finally, various circular RNAs that are produced from spliced introns and exons have been reported. For example, excised, self-spliced group I and group II introns are ligated to form circular RNAs with either 3\u0374,5\u0374-phosphodiester bonds or 2\u0374,5\u0374-phosphodiester bonds. Excised tRNA introns are removed by tRNA splicing enzymes and then undergo 3\u0374,5\u0374-phosphodiester ligation in Archaea and Metazoa. Circular RNAs derived from lariat introns have been reported in human cells and in Xenopus tropicalis<\/i>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner]\t\t

\n\t\t\tNeed a quotation?<\/span>\r\n\r\n\r\n<\/svg>\r\n<\/a>\n\t\t<\/div>\n\t\t\n\t[vc_row_inner et_max_width=”” et_column_align=”align-center” content_placement=”middle”][vc_column_inner offset=”vc_col-lg-6″][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_column_align=”align-center” css=”.vc_custom_1579113617984{padding-top: 60px !important;padding-bottom: 60px !important;}”][vc_column width=”1\/2″ animation=”animation bottom-to-top”][vc_tta_accordion active_section=”1″][vc_tta_section title=”Specifications” tab_id=”1557543124640-60cb9bb3-2cc8″][vc_column_text]\n
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  • 150bp pair-end sequencing reads<\/li>\n
  • rRNA depletion and RNase linearRNA digestion library service<\/li>\n
  • Standard output 30-40 Million reads per sample<\/li>\n
  • Clean data and bioinformatics analysis are available
    \nin standard file formats<\/li>\n
  • Available data storage and bioinformatics applications<\/li>\n
  • Cloud-based data storage and delivery system<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_tta_section][vc_tta_section title=”Sample Requirements” tab_id=”1557543124710-e0287025-a0eb”][vc_column_text]\n\n\n\n\n\n\n
    Total RNA of Animals<\/td>\nStrongly Recommended<\/td>\n15 \u03bcL – 100 \u03bcL<\/td>\nm\u226510 \u03bcg<\/td>\nc\u226540ng\/\u03bcL, RIN\u22657.0, 28S\/18S\u22651.0<\/td>\n<\/tr>\n
    Required<\/td>\n5\u03bcg\u2264m<10\u03bcg<\/td>\n<\/tr>\n
    Total RNA of Plant<\/td>\nStrongly Recommended<\/td>\n15 \u03bcL – 100 \u03bcL<\/td>\nm\u226510 \u03bcg<\/td>\nc\u226540ng\/\u03bcL, RIN\u22656.5, 28S\/18S\u22651.0<\/td>\n<\/tr>\n
    Required<\/td>\n5\u03bcg\u2264m<10\u03bcg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][\/vc_tta_section][vc_tta_section title=”Standart Bioinformatics Analysis” tab_id=”1684783447897-d707e395-dd7f”][vc_column_text]\n
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    • Quality control and statistics of Reads<\/li>\n
    • Mapping to genome<\/li>\n
    • Localization of circRNA on genome<\/li>\n
    • Overview of Statistics of circRNA typies<\/li>\n
    • FPKM Interval (FI) of circRNA expression<\/li>\n
    • Density of circRNA expression<\/li>\n
    • CircRNA expression profiling<\/li>\n
    • Statistics of back splicing junctions<\/li>\n
    • Profiling of differentially expressed circRNA<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_tta_section][\/vc_tta_accordion][\/vc_column][\/vc_row][vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113623784{padding-top: 60px !important;background-color: #f6f6f6 !important;}”][vc_column][vc_row_inner et_column_align=”align-center” content_placement=”middle”][vc_column_inner animation=”animation bottom-to-top” offset=”vc_col-lg-6″][vc_column_text css=”.vc_custom_1693853737154{padding-right: 10% !important;padding-left: 10% !important;}”]\n

      DNBSEQ\u2122 Sequencing Technology<\/h2>\n

      BGI’s RNA Sequencing services are typically executed with proprietary DNBSEQ\u2122 sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ\u2122 offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ\u2122 platforms.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner et_max_width=”” et_column_align=”align-center” content_placement=”middle”][vc_column_inner animation=”animation right-to-left” offset=”vc_col-lg-6″]\t

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      \n\t\t\t\t\t\"\"\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t<\/div>\n [\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

      [vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}”][vc_column css=”.vc_custom_1684760689814{background-color: #f6f6f6 !important;}”][vc_row_inner][vc_column_inner animation=”animation bottom-to-top”][vc_column_text css=”.vc_custom_1684924191248{margin-bottom: 0px !important;}”] circRNAs are a novel class of abundant, stable and ubiquitous RNAs. circRNAs are distinguished from mRNAs in that they lack poly (A) tails and 5\u0374 caps, and are resistant to exonuclease treatment.…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3900","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/3900","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3900"}],"version-history":[{"count":19,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/3900\/revisions"}],"predecessor-version":[{"id":4528,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/3900\/revisions\/4528"}],"wp:attachment":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3900"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}