{"id":3871,"date":"2023-05-23T20:30:02","date_gmt":"2023-05-23T20:30:02","guid":{"rendered":"http:\/\/www.geneethic.com.tr\/?page_id=3871"},"modified":"2026-04-03T07:01:57","modified_gmt":"2026-04-03T07:01:57","slug":"iso-seq-pacbio-transcriptome-sequencing","status":"publish","type":"page","link":"https:\/\/www.geneethic.com.tr\/?page_id=3871","title":{"rendered":"Iso-Seq \/ PacBio Transcriptome Sequencing"},"content":{"rendered":"[vc_row][vc_column][vc_column_text]\n
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      \u201cIsoform Sequencing\u201d (Iso-seq) developed by Pacific Biosciences (PacBio), is based on long-read sequencing technology and allows researchers to identify new isoforms with extraordinary precision. Contact us today with any questions or for a no obligation quote.<\/p>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_full_width=”true” et_row_padding=”true” css=”.vc_custom_1579113590633{padding-top: 60px !important;background-color: #f6f6f6 !important;}”][vc_column][vc_row_inner et_column_align=”align-center” content_placement=”middle”][vc_column_inner width=”1\/2″ animation=”animation bottom-to-top” offset=”vc_col-lg-6″][vc_column_text css=”.vc_custom_1684873930805{margin-bottom: 0px !important;}”]Isoform Sequencing (Iso-seq) developed by Pacific Biosciences (PacBio), is based on long-read sequencing technology. The unique long-read sequencing feature allows this method to identify new isoforms with extraordinary precision. The Iso-Seq application generates full-length cDNA sequences \u2014 from the 5\u2019 end of transcripts to the poly-A tail \u2014 eliminating the need for transcriptome reconstruction using isoform-inference algorithms. The Iso-Seq method provides accurate information about alternatively spliced exons and transcriptional start sites. It also reveals information about poly-adenylation sites for transcripts across the full complement of isoforms within targeted genes or the entire transcriptome.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″ animation=”animation bottom-to-top”][vc_column_text css=”.vc_custom_1775198406923{margin-bottom: 0px !important;}”]RNA sequencing has become the most frequently used method for the majority of researchers conducting gene expression profiling. However, it is difficult to obtain a complete picture of the transcriptome because short reads cannot accurately assemble complex transcripts.<\/p>\n

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