{"id":1450,"date":"2019-04-19T04:23:23","date_gmt":"2019-04-19T04:23:23","guid":{"rendered":"https:\/\/goya.everthemes.com\/demo-basic\/?page_id=1450"},"modified":"2024-11-12T11:46:50","modified_gmt":"2024-11-12T11:46:50","slug":"lookbook","status":"publish","type":"page","link":"https:\/\/www.geneethic.com.tr\/?page_id=1450","title":{"rendered":"High Throughput Single Cell RNA-Seq"},"content":{"rendered":"[vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column css=&#8221;.vc_custom_1684760689814{background-color: #f6f6f6 !important;}&#8221;][vc_row_inner][vc_column_inner animation=&#8221;animation bottom-to-top&#8221;][vc_single_image image=&#8221;4850&#8243; img_size=&#8221;medium&#8221; alignment=&#8221;center&#8221;][vc_column_text css=&#8221;.vc_custom_1731411998723{margin-bottom: 0px !important;}&#8221;]\n<p style=\"text-align: center;\">Cell populations are heterogeneous and unsynchronized in their characteristics. Traditional bulk RNA sequencing generates comprehensive information but does not provide insight into variable expression between cells. High throughput single cell RNA-Seq provides a scalable solution for cell characterization and gene expression profiling of hundreds to millions of cells. High throughput single cell RNA-Seq technology is able to handle a large quantity of single cells fast and accurately, providing research solutions across a wide variety of applications including cancer, neurology, and immunology.<\/p>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113590633{padding-top: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column][vc_row_inner et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner width=&#8221;1\/2&#8243; animation=&#8221;animation bottom-to-top&#8221; offset=&#8221;vc_col-lg-6&#8243;][vc_column_text css=&#8221;.vc_custom_1699875411228{margin-bottom: 0px !important;}&#8221;]\n<div class=\"md:px-[13%] px-[5%] mb-14\">\n<div>\n<h4 class=\"style_seve_content__QlWY2\">The major applications of single cell sequencing include:<\/h4>\n<\/div>\n<\/div>\n[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243; animation=&#8221;animation bottom-to-top&#8221;][vc_column_text css=&#8221;.vc_custom_1699875468785{margin-bottom: 0px !important;}&#8221;]\n<div class=\"flex-1\">\n<ul>\n<li class=\"text-lg text-[#3ba792] mb-4\">Discovering new cells and cell subtypes<\/li>\n<li>\n<div class=\"style_seve_content__QlWY2\">Studying tissue and cell group heterogeneity<\/div>\n<\/li>\n<li>\n<div class=\"style_seve_content__QlWY2\">Studying tumor-immune cell interaction<\/div>\n<\/li>\n<li>\n<div class=\"style_seve_content__QlWY2\">Searching for marker genes for drug development<\/div>\n<\/li>\n<\/ul>\n<\/div>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner]\t\t<div id=\"et-button-6a9e921ea504b\" class=\"et_btn_align_center \">\n\t\t\t<a href=\"http:\/\/www.geneethic.com.tr\/?page_id=2221\" class=\" et_btn button et_btn_md solid color-  arrow-enabled \"  role=\"button\" title=\"Contact_Geneethic\" ><span>Need a quotation?<\/span><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\"\r\n\t width=\"30px\" height=\"18px\" viewBox=\"0 0 30 18\" enable-background=\"new 0 0 30 18\" xml:space=\"preserve\">\r\n<path class=\"handle\" d=\"M20.305,16.212c-0.407,0.409-0.407,1.071,0,1.479s1.068,0.408,1.476,0l7.914-7.952c0.408-0.409,0.408-1.071,0-1.481\r\n\tl-7.914-7.952c-0.407-0.409-1.068-0.409-1.476,0s-0.407,1.071,0,1.48l7.185,7.221L20.305,16.212z\"\/>\r\n<path class=\"bar\" fill-rule=\"evenodd\" clip-rule=\"evenodd\" d=\"M1,8h28.001c0.551,0,1,0.448,1,1c0,0.553-0.449,1-1,1H1c-0.553,0-1-0.447-1-1\r\n\tC0,8.448,0.447,8,1,8z\"\/>\r\n<\/svg>\r\n<\/a>\n\t\t<\/div>\n\t\t\n\t[vc_row_inner et_max_width=&#8221;&#8221; et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner offset=&#8221;vc_col-lg-6&#8243;]\t<div id=\"et-image-290\" class=\"et-image aligncenter   small-shadow et_image_link\">\n\t\t<div class=\"et-image-inner animation left-to-right\">\n\t\t\t\t\t\t\t<div class=\"et-image-thumb \">\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t<\/div>\n  [\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row et_column_align=&#8221;align-center&#8221; css=&#8221;.vc_custom_1579113617984{padding-top: 60px !important;padding-bottom: 60px !important;}&#8221;][vc_column width=&#8221;1\/2&#8243; animation=&#8221;animation bottom-to-top&#8221;][vc_tta_accordion active_section=&#8221;1&#8243;][vc_tta_section title=&#8221;Specifications&#8221; tab_id=&#8221;1557543124640-60cb9bb3-2cc8&#8243;][vc_column_text]\n<ul>\n<li>Guaranteed \u226590% of bases with quality score of \u2265Q20<\/li>\n<li>DNBSEQ\u2122 PE100 or PE150 options<\/li>\n<li>Raw data and bioinformatics analysis are available in standard file formats<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Sample Requirements&#8221; tab_id=&#8221;1557543124710-e0287025-a0eb&#8221;][vc_column_text]\n<h5 class=\"text-lg text-[#3ba792] mb-4\">Cell line\/Tissue\/PBMC:<\/h5>\n<div>\n<div class=\"style_seve_content__QlWY2\">\n<ul>\n<li>After sample processing, freeze cells at a final concentration of 1&#215;10^6 cells\/ml in cryopreserved tubes (Cell freezing medium: 90% FBS+10% DMSO)<\/li>\n<\/ul>\n<\/div>\n<\/div>\n[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Standart Bioinformatics Analysis&#8221; tab_id=&#8221;1684783447897-d707e395-dd7f&#8221;][vc_column_text]\n<ul>\n<li>Sequencing Result<\/li>\n<li>Comparison Result<\/li>\n<li>Quantitative Analysis<\/li>\n<li>Data QC<\/li>\n<li>Cell Cluster<\/li>\n<li>Differential Gene Identification Between Samples<\/li>\n<li>Cell Cluster Annotation<\/li>\n<li>Marker Gene\/Differentially Expressed Gene GO Pathway<\/li>\n<li>Marker Gene\/Differentially Expressed Gene KEGG Pathway Fnrichment<\/li>\n<li>RNA Velocity<\/li>\n<li>CellPhone DB<\/li>\n<li>Pseudotemporal Ordering<\/li>\n<li>Customized Cell Cluster Analysis<\/li>\n<li>Customized Cell Cluster Annotation By Database of your choice<\/li>\n<li>Gene-Protein Interaction Analysis<\/li>\n<li>Gene Function Enrichment Analysis<\/li>\n<li>Dynamic Interactive Analysis of Cell Type And Cell Trajectory Analysis<\/li>\n<li>Different Dimension Analysis of Differential Gene Between Cell Types\/Samples<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_tta_section][\/vc_tta_accordion][vc_column_text animation=&#8221;animation bottom-to-top&#8221;]\n<p style=\"text-align: center;\"><strong><a href=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/High-Throughput-Single-Cell-RNA-Seq-Catalog-2022.10_2.pdf\">High Throughput Single Cell RNA-Seq Catalog<\/a><\/strong><\/p>\n<p style=\"text-align: center;\"><a href=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/High-Throughput-Single-Cell-RNA-Seq-Catalog-2022.10_2.pdf\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4369 alignnone\" src=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-300x300.webp\" alt=\"\" width=\"75\" height=\"75\" srcset=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-300x300.webp 300w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-1024x1024.webp 1024w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-150x150.webp 150w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280-768x768.webp 768w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/11\/download-6155763_1280.webp 1280w\" sizes=\"auto, (max-width: 75px) 100vw, 75px\" \/><\/a><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113623784{padding-top: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column][vc_row_inner et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner animation=&#8221;animation bottom-to-top&#8221; offset=&#8221;vc_col-lg-6&#8243;][vc_column_text css=&#8221;.vc_custom_1684782487573{padding-right: 10% !important;padding-left: 10% !important;}&#8221;]\n<h2 style=\"text-align: center;\">Unique DNBSEQ\u2122 Sequencing Technology<\/h2>\n<div>\n<div class=\"sm:leading-normal leading-loose text-base text-text-600\"><\/div>\n<\/div>\n<p style=\"text-align: center;\">BGI&#8217;s sequencing services are typically executed with proprietary DNBSEQ\u2122 sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. DNBSEQ\u2122 offers advantages in terms of lower amplification error rates and much lower duplication rates. In addition, studies have shown the lower index hopping rate in DNBSEQ\u2122 platforms.<\/p>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner et_max_width=&#8221;&#8221; et_column_align=&#8221;align-center&#8221; content_placement=&#8221;middle&#8221;][vc_column_inner animation=&#8221;animation right-to-left&#8221; offset=&#8221;vc_col-lg-6&#8243;]\t<div id=\"et-image-344\" class=\"et-image     et_image_link\">\n\t\t<div class=\"et-image-inner animation bottom-to-top\">\n\t\t\t\t\t\t\t<div class=\"et-image-thumb \">\n\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1522\" height=\"582\" src=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede.png\" class=\"attachment-full\" alt=\"\" srcset=\"https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede.png 1522w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-300x115.png 300w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-1024x392.png 1024w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-768x294.png 768w, https:\/\/www.geneethic.com.tr\/wp-content\/uploads\/2023\/05\/dedede-150x57.png 150w\" sizes=\"auto, (max-width: 1522px) 100vw, 1522px\" \/>\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t<\/div>\n  [\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"<p>[vc_row et_full_width=&#8221;true&#8221; et_row_padding=&#8221;true&#8221; css=&#8221;.vc_custom_1579113527778{padding-top: 120px !important;padding-bottom: 60px !important;background-color: #f6f6f6 !important;}&#8221;][vc_column css=&#8221;.vc_custom_1684760689814{background-color: #f6f6f6 !important;}&#8221;][vc_row_inner][vc_column_inner animation=&#8221;animation bottom-to-top&#8221;][vc_single_image image=&#8221;4850&#8243; img_size=&#8221;medium&#8221; alignment=&#8221;center&#8221;][vc_column_text css=&#8221;.vc_custom_1731411998723{margin-bottom: 0px !important;}&#8221;] Cell populations are heterogeneous and unsynchronized in their characteristics. Traditional bulk RNA sequencing generates comprehensive information but does not provide insight into variable expression between cells. High throughput single&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1450","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/1450","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1450"}],"version-history":[{"count":11,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/1450\/revisions"}],"predecessor-version":[{"id":4878,"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/1450\/revisions\/4878"}],"wp:attachment":[{"href":"https:\/\/www.geneethic.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1450"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}